Isolation of Saccharomyces cerevisiae mitochondrial formyltetrahydrofolic acid:methionyl-tRNA transformylase and the hybridization of mitochondrial fMet-tRNA with mitochondrial DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 2 ·1971-02-00 ·Pages 294-8

Halbreich A, Rabinowitz M

Abstract

Formyltetrahydrofolic acid:methionyl-tRNA transformylase was isolated from Saccharomyces cerevisiae mitochondria and used to prepare yeast mitochondrial [(3)H]formylmethionyl-tRNA. This fMet-tRNA hybridizes with mitochondrial DNA but not with yeast nuclear or E. coli DNA. Unlabeled mitochondrial, but not extramitochondrial, tRNA competes in this reaction. tRNA was eluted from the hybrid and found to contain the label almost exclusively in fMet-tRNA. Yeast cytoplasmic fMet-tRNA formylated with Escherichia coli enzyme, and E. coli fMet-tRNA, do not hybridize with mitochondrial DNA. It is concluded that yeast mitochondrial tRNA(fMet) is a gene product of the mitochondrial genome.

MeSH Terms

DNA

Chemicals

DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Halbreich A

Rabinowitz M

References (17)

17 references, click to expand

The formation of N-formyl-methionyl-sRNA.
J Mol Biol. 1965 Nov;14(1):63-70 PMID: 5327658
Informational role of mitochondrial DNA studied by hybridization with different classes of RNA in yeast.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1065-72 PMID: 5233836
A distinct class of ribosomal RNA components in yeast mitochondria as revealed by gradient centrifugation and by DNA-RNA-hybridization.
Hoppe Seylers Z Physiol Chem. 1967 Dec;348(12):1701-4 PMID: 4870503
Transfer RNA coded by the T4 bacteriophage genome.
Proc Natl Acad Sci U S A. 1968 Sep;61(1):114-21 PMID: 4880604
Studies on polynucleotides. XCI. Yeast methionine transfer ribonucleic acid: purification, properties, and terminal nucleotide sequences.
J Biol Chem. 1969 Mar 10;244(5):1104-13 PMID: 4886181
Separation and characterization of a satellite DNA from a yeast cytoplasmic "petite" mutant.
J Mol Biol. 1968 Nov 14;37(3):493-505 PMID: 5719220
A convenient procedure for preparing transfer ribonucleic acid from Escherichia coli.
Biochim Biophys Acta. 1969 Apr 22;179(2):297-307 PMID: 4890595
Synthesis of mitochondrial and nuclear DNA in anerobically grown yeast during the development of mitochondrial function in response to oxygen.
J Mol Biol. 1969 May 14;41(3):381-400 PMID: 5803290
Cytoplasmic methionine transfer RNAs from eukaryotes.
Nature. 1970 May 16;226(5246):607-10 PMID: 4315548
Mitochondrial nucleic acids and their relation to the biogenesis of mitochondria.
Physiol Rev. 1970 Jul;50(3):376-427 PMID: 4912905
Initiation of protein synthesis in rabbit reticulocytes.
Biochem Biophys Res Commun. 1970 Jul 27;40(2):402-7 PMID: 4919961
Mitochondrial genetics. I. Methodology and phenomenology.
Symp Soc Exp Biol. 1970;24:449-96 PMID: 5516343
N-formylmethionyl transfer RNA in mitochondria from yeast and rat liver.
J Mol Biol. 1968 Dec 14;38(2):241-3 PMID: 5760639
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
N-FORMYL-METHIONYL-S-RNA.
J Mol Biol. 1964 Jun;8:835-40 PMID: 14187409
Isolation and fractionation of nucleic acids.
Prog Nucleic Acid Res Mol Biol. 1964;3:1-31 PMID: 5318915
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.2.294

Published

1971-02-00

Pages

294-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388921

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.2.294 PMC Full Text View journal details

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Isolation of Saccharomyces cerevisiae mitochondrial formyltetrahydrofolic acid:methionyl-tRNA transformylase and the hybridization of mitochondrial fMet-tRNA with mitochondrial DNA.

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