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PMID: 4217628 Published · ppublish English Journal Article

Mitochondrial and cytoplasmic ribosomes. Distinguishing characteristics and a requirement for the homologous ribosomal salt-extractable fraction for protein synthesis.

The Biochemical journal ·Vol. 140 ·No. 1 ·1974-04-00 ·Pages 73-8

Avadhani NG, Buetow DE

Abstract

1. Mitochondrial and cytoplasmic ribosomes of Euglena gracilis differ in their total RNA and protein content. 2. Mitochondrial ribosomes dissociate to subunits at higher Mg(2+) concentrations than do cytoplasmic ribosomes. 3. A separable 5S RNA is obtained from cytoplasmic and chloroplast ribosomes, but not from mitochondrial ribosomes. 4. For protein-synthesizing activity with a natural mRNA, mitochondrial ribosomes use tRNA from any cell compartment and are partly active with supernatant enzymes from cytoplasm. Cytoplasmic ribosomes are partly active with enzymes and tRNA from mitochondria or chloroplasts. 5. Both mitochondrial and cytoplasmic ribosomes show high specificity for the homologous salt-extractable ribosomal fraction for protein-synthesizing activity.

MeSH Terms
Cell Fractionation Chloroplasts/metabolism Cytoplasm/metabolism Euglena gracilis/metabolism Magnesium/pharmacology Mitochondria/metabolism Protein Biosynthesis RNA, Ribosomal/metabolism Ribosomes/drug effects,metabolism,ultrastructure
Chemicals
RNA, Ribosomal Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Avadhani N G
Buetow D E
References (34)
34 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1400073
Published
1974-04-00
Pages
73-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1167972
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1400073 PMC Full Text View journal details
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