A factor promoting the initiation of globin synthesis in a rabbit reticulocyte cell-free system.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 1 ·1969-05-00 ·Pages 129-34

Fuhr JE, London IM, Grayzel AI

Abstract

The synthesis of globin has been studied in a reticulocyte cell-free system in which the initiation of polypeptide chain synthesis can proceed for as long as ten minutes of incubation. With this system, we have found evidence for a soluble factor in the reticulocyte that stimulates protein synthesis and appears to act by promoting initiation.

MeSH Terms

Amino Acids/metabolism Animals Blood Proteins/biosynthesis Carbon Isotopes Cell-Free System Centrifugation, Density Gradient Globins/biosynthesis Hemoglobins/biosynthesis Lysine/metabolism Methods Peptide Biosynthesis Rabbits Reticulocytes/metabolism

Chemicals

Amino Acids Blood Proteins Carbon Isotopes Hemoglobins Globins Lysine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Fuhr J E

London I M

Grayzel A I

References (15)

15 references, click to expand

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Studies on ribosomes from reticulocytes.
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Arch Biochem Biophys. 1968 May;125(2):542-7 PMID: 4872321
The stimulation of globin synthesis by heme.
Proc Natl Acad Sci U S A. 1966 Mar;55(3):650-5 PMID: 5221248
The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell-free reticulocyte system.
J Mol Biol. 1966 Oct 28;21(1):51-69 PMID: 5969765
Evidence for aminoacyl-tRNA binding, peptide bond synthesis, and translocase activities in the aminoacyl transfer reaction.
Arch Biochem Biophys. 1968 May;125(2):497-505 PMID: 5656804
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J Mol Biol. 1964 Aug;9:558-75 PMID: 14202286
Isolation of a protein fraction from reticulocyte ribosomes required for de novo synthesis of hemoglobin.
Arch Biochem Biophys. 1968 May;125(2):632-46 PMID: 5656813
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Proc Natl Acad Sci U S A. 1966 Aug;56(2):701-8 PMID: 5229987
A multiple ribosomal structure in protein synthesis.
Proc Natl Acad Sci U S A. 1963 Jan 15;49:122-9 PMID: 13998950
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.63.1.129

Published

1969-05-00

Pages

129-34

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC534011

Subset

External Links

PubMed source DOI: 10.1073/pnas.63.1.129 PMC Full Text View journal details

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A factor promoting the initiation of globin synthesis in a rabbit reticulocyte cell-free system.

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