Clonal nature of the immune response to phosphorylcholine. IV. Idiotypic uniformity of binding site-associated antigenic determinants among mouse antiphosphorylcholine antibodies.

The Journal of experimental medicine ·Vol. 140 ·No. 3 ·1974-09-01 ·Pages 673-86

Claflin JL, Davie JM

Abstract

A new idiotypic determinant(s) on mouse anti-PC antibodies is described. Antibodies to the determinant(s) were raised in rabbits by immunization with HOPC 8, a PC-binding myeloma protein, and were isolated from HOPC 8 immunoadsorbent by elution with PC. These antibodies react with binding site determinants on anti-PC antibodies raised in all 15 inbred mouse strains tested regardless of histocompatibility or allotype, but fail to react with antibodies of other specificities or with anti-PC antibodies raised in other rodent species. These results correlate closely with other studies which show similar binding specificity of anti-PC antibodies raised in 17 different strains of mice. The site-associated idiotypic determinant(s) is clearly distinct from that detected by mouse anti-HOPC 8 antisera. This latter determinant(s) is present on anti-PC antibodies of only a few strains of mice and may not be in the binding site.

MeSH Terms

Animals Antibodies, Neoplasm Antibody Specificity Binding Sites, Antibody Cell Line Choline/analysis,immunology Dinitrophenols/immunology Epitopes Haptens Hemagglutination Tests Iodine Radioisotopes Mice Mice, Inbred Strains Myeloma Proteins/immunology Organophosphorus Compounds/immunology Phosphatidylcholines/immunology Plasmacytoma/immunology Radioimmunoassay Species Specificity

Chemicals

Antibodies, Neoplasm Dinitrophenols Epitopes Haptens Iodine Radioisotopes Myeloma Proteins Organophosphorus Compounds Phosphatidylcholines Choline

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Claflin J L

Davie J M

References (36)

36 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.140.3.673

Published

1974-09-01

Pages

673-86

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2139606

Subset

External Links

PubMed source DOI: 10.1084/jem.140.3.673 PMC Full Text View journal details

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Clonal nature of the immune response to phosphorylcholine. IV. Idiotypic uniformity of binding site-associated antigenic determinants among mouse antiphosphorylcholine antibodies.

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