Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 235-9

Carson D, Weigert M

Abstract

The idiotype of the mouse myeloma protein with anti-alpha-1,3 dextran activity, J558, has been characterized by a solid-phase radioimmunoassay. The idiotype of the J558 protein depends on a specific light and heavy chain interaction and is altered in the presence of the hapten, nigerose. Cross-reacting idiotypes were found on another mouse myeloma protein with alpha1,3 dextran specificity, normal anti-dextran antibody, and certain reconstructed myeloma proteins composed of the J558 heavy chain and heterologous light chains.

MeSH Terms

Amino Acid Sequence Animals Antibody Specificity Antigen-Antibody Reactions/drug effects Binding Sites, Antibody Binding, Competitive Cross Reactions Dextrans Disaccharides/pharmacology Epitopes Genetic Code Haptens Iodine Isotopes Maltose/pharmacology Mice/immunology Myeloma Proteins/analysis,isolation & purification Neoplasms, Experimental Plasmacytoma Radioimmunoassay

Chemicals

Dextrans Disaccharides Epitopes Haptens Iodine Isotopes Myeloma Proteins Maltose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Carson D

Weigert M

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.1.235

Published

1973-01-00

Pages

235-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433222

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.1.235 PMC Full Text View journal details

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Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody.

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