Detection of idiotypic cross-reactions among streptococcal antisera from related rabbits.

The Journal of experimental medicine ·Vol. 138 ·No. 3 ·1973-09-01 ·Pages 522-37

Kindt TJ, Seide RK, Bokisch VA, Krause RM

Abstract

Idiotypic cross-reactions among antibodies to Group C streptococcal carbohydrate were studied using idiotypic antisera prepared in allotypically matched rabbits. Antibodies with idiotypic cross-specificity to one proband antibody were detected in 58% of the antisera from related rabbits, while approximately 1% of nonrelated rabbits produced antibody with this specificity. The cross-specificity was related to the group a (V(H)) allotype of 133 rabbits tested with only one exception. Studies utilizing antisera against a second proband antibody failed to detect antibodies with idiotypic cross-reactivity among the same group of related rabbits. This result emphasizes the variation in expression of idiotypic determinants of antibodies. It was further shown that the presence of anti-IgG's in the streptococcal antisera interfere with the detection of idiotypic cross-reactions. These anti-IgG's masked the presence of antibodies with idiotypic cross-specificity when inhibition of precipitation tests were used for their detection.

MeSH Terms

Animals Antibodies, Bacterial/analysis Antibody Specificity Cross Reactions Genotype Hemagglutination Inhibition Tests Hemagglutination Tests Immune Sera Immunoglobulin G Iodine Isotopes Polysaccharides, Bacterial Precipitin Tests Rabbits/immunology Streptococcus/immunology

Chemicals

Antibodies, Bacterial Immune Sera Immunoglobulin G Iodine Isotopes Polysaccharides, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kindt T J

Seide R K

Bokisch V A

Krause R M

References (22)

22 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.138.3.522

Published

1973-09-01

Pages

522-37

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2139422

Subset

External Links

PubMed source DOI: 10.1084/jem.138.3.522 PMC Full Text View journal details

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Detection of idiotypic cross-reactions among streptococcal antisera from related rabbits.

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