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PMID: 4131516 Published · ppublish English Journal Article

Genetics of a new IgVH (T15 idiotype) marker in the mouse regulating natural antibody to phosphorylcholine.

The Journal of experimental medicine ·Vol. 139 ·No. 4 ·1974-04-01 ·Pages 983-1001

Lieberman R, Potter M, Mushinski EB, Humphrey W, Rudikoff S

Abstract

The idiotype present on the Fab of a phosphorylcholine-binding IgA myeloma protein TEPC 15 (T15) of BALB/c origin was found in normal serum of BALB/c mice. Molecules carrying the T15 idiotype in normal serum could be adsorbed with Sepharose phosphorylcholine beads and R36A pneumococci. The T15 idiotype is absent in germ-free BALB/c but appears when the mice are conventionalized. A survey of normal sera of inbred strains for the T15 idiotype showed it to be present in BALB/c, 129, C57L, C58, and ST and absent or in low levels in CBA, C3H, C57BL/6, C57BL/Ka, C57BL/10, SJL, B10.D2, DBA/2, RIII, A, AL, AKR, NZB, and NH inbred strains of mice. The T15 idiotype is associated with some but not all strains carrying the IgC(H) allotypes found in BALB/c. Linkage of genes controlling the T15 idiotype in normal serum to the IgC(H) locus of BALB/c was demonstrated in F(2) progeny of a BALB/c and C57BL cross, Bailey's recombinant inbred strains, C x BD, C x BE, C x BG, C x BH, C x BI, C x BJ, C x BK, and CB20 congenic strains. Among these strains, only those possessing the IgC(H) locus of BALB/c including the F(2) progeny consisting of BALB/c homozygotes and BALB/c/C57BL heterozygotes and C x BG and C x BJ recombinants showed the T15 idiotype.

MeSH Terms
Animals Antibodies Antibody Specificity Binding Sites, Antibody Choline Epitopes Erythrocytes/immunology Genes Genetic Linkage Haptens Hemagglutination Hemagglutination Inhibition Tests Immunogenetics Immunoglobulin A Mice Mice, Inbred Strains Myeloma Proteins Organophosphorus Compounds Phenotype Recombination, Genetic Sheep/immunology
Chemicals
Antibodies Epitopes Haptens Immunoglobulin A Myeloma Proteins Organophosphorus Compounds Choline
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lieberman R
Potter M
Mushinski E B
Humphrey W
Rudikoff S
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31 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.139.4.983
Published
1974-04-01
Pages
983-1001
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139576
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.139.4.983 PMC Full Text View journal details
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