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PMID: 4040134 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Antigenic polymorphism of the LamB protein among members of the family Enterobacteriaceae.

Journal of bacteriology ·Vol. 163 ·No. 1 ·1985-07-00 ·Pages 106-10

Bloch MA, Desaymard C

Abstract

In this study we demonstrate that most members of the family Enterobacteriaceae possess a maltose-inducible outer membrane protein homologous to the LamB protein of Escherichia coli K-12. These proteins react with polyclonal antibodies raised against the LamB protein of E. coli K-12. We compared the antigenic structure of the LamB protein in members of the family Enterobacteriaceae with six monoclonal antibodies raised against the LamB protein of E. coli K-12. Four of them reacted with epitopes located at the outer face of the membrane, and two reacted with epitopes located at the inner face of the membrane. A great degree of variability was observed for the external epitopes. Even in a single species, such as E. coli, an important polymorphism was present. In contrast, the internal epitopes were more conserved.

MeSH Terms
Antigens, Bacterial/analysis Bacterial Outer Membrane Proteins/immunology Biological Evolution Cross Reactions Enterobacteriaceae/immunology Molecular Weight Polymorphism, Genetic Porins Receptors, Virus/immunology Species Specificity
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Porins Receptors, Virus maltoporins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bloch M A
Desaymard C
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.1.106-110.1985
Published
1985-07-00
Pages
106-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219086
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.163.1.106-110.1985 PMC Full Text View journal details
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