Home LiteratureArticle Details
PMID: 4032536 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions.

Journal of virology ·Vol. 56 ·No. 1 ·1985-10-00 ·Pages 227-39

Rice CM, Franke CA, Strauss JH, Hruby DE

Abstract

We have obtained a vaccinia virus recombinant which contains a complete cDNA copy of the 26S RNA of Sindbis virus within the thymidine kinase gene of the vaccinia virus genome. This recombinant constitutively transcribed the Sindbis sequences throughout the infectious cycle, reflecting the dual early-late vaccinia promoter used in this construction. The Sindbis-derived transcripts were translationally active, giving rise to both precursor and mature structural proteins of Sindbis virus, including the capsid protein (C), the precursor of glycoprotein E2 (PE2), and the two mature envelope glycoproteins (E1 and E2). These are the same products translated from the 26S mRNA during Sindbis infection, and thus these proteins were apparently cleaved, glycosylated, and transported in a manner analogous to that seen during authentic Sindbis infections. By using epitope-specific antibodies, it was possible to demonstrate that recombinant-derived proteins were incorporated into Sindbis virions during coinfections with monoclonal antibody-resistant Sindbis variants. These results suggest that all the information necessary to specify the proper biogenesis of Sindbis virus structural proteins resides within the 26S sequences and that vaccinia may provide an appropriate system for using DNA molecular genetic manipulations to unravel a variety of questions pertinent to RNA virus replication.

MeSH Terms
Biological Transport DNA, Recombinant Gene Expression Regulation Glycoproteins/genetics Molecular Weight Morphogenesis Plasmids Protein Processing, Post-Translational Sindbis Virus/genetics,ultrastructure Time Factors Transcription, Genetic Vaccinia virus/genetics Viral Proteins/genetics Virion/ultrastructure Virus Replication
Chemicals
DNA, Recombinant Glycoproteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rice C M
Franke C A
Strauss J H
Hruby D E
References (54)
54 references, click to expand
  1. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  2. Evidence for an autoprotease activity of sindbis virus capsid protein.
    Virology. 1978 Oct 15;90(2):366-9 PMID: 726255
  3. Vaccinia virus replication. I. Requirement for the host-cell nucleus.
    J Virol. 1979 Feb;29(2):705-15 PMID: 107327
  4. Two small virus-specific polypeptides are produced during infection with Sindbis virus.
    J Virol. 1979 Mar;29(3):1186-95 PMID: 448798
  5. Cellular differences in the molecular mechanisms of vaccinia virus host range restriction.
    J Gen Virol. 1980 Apr;47(2):485-8 PMID: 6767809
  6. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  7. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  8. Detection and characterization of mouse mammary tumor virus cell surface antigens: estimation of antigen abundance by protein A assay.
    J Virol. 1980 Sep;35(3):876-87 PMID: 6252344
  9. Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins.
    Nature. 1980 Nov 20;288(5788):236-41 PMID: 6985476
  10. In vitro transcription of the inverted terminal repetition of the vaccinia virus genome: correspondence of initiation and cap sites.
    J Virol. 1981 Feb;37(2):738-47 PMID: 6452534
  11. Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2062-6 PMID: 6941270
  12. Cell-free synthesis of enzymatically active vaccinia virus thymidine kinase.
    Virology. 1981 Sep;113(2):594-601 PMID: 7269256
  13. Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1210-4 PMID: 6280173
  14. Molecular genetics of vaccinia virus: demonstration of marker rescue.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1593-6 PMID: 6951197
  15. Association of sindbis virion glycoproteins and their precursors.
    J Mol Biol. 1982 Jan 15;154(2):325-48 PMID: 7077663
  16. Restriction enzyme mapping of vaccinia virus DNA.
    J Virol. 1982 Jul;43(1):136-49 PMID: 6286993
  17. Expression of the structural proteins of Semliki Forest virus from cloned cDNA microinjected into the nucleus of baby hamster kidney cells.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4525-9 PMID: 6956877
  18. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927-31 PMID: 6289324
  19. Vaccinia virus: a selectable eukaryotic cloning and expression vector.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9 PMID: 6296831
  20. Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen.
    Nature. 1983 Apr 7;302(5908):490-5 PMID: 6835382
  21. Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations.
    J Virol. 1983 May;46(2):530-7 PMID: 6842679
  22. Expression of Semliki Forest virus proteins from cloned complementary DNA. I. The fusion activity of the spike glycoprotein.
    J Cell Biol. 1983 Sep;97(3):644-51 PMID: 6688423
  23. Expression of Semliki Forest virus proteins from cloned complementary DNA. II. The membrane-spanning glycoprotein E2 is transported to the cell surface without its normal cytoplasmic domain.
    J Cell Biol. 1983 Sep;97(3):652-8 PMID: 6688424
  24. Construction of live vaccines by using genetically engineered poxviruses: biological activity of recombinant vaccinia virus expressing influenza virus hemagglutinin.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5364-8 PMID: 6310573
  25. Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
    Methods Enzymol. 1983;100:255-66 PMID: 6194404
  26. Protective monoclonal antibodies define maturational and pH-dependent antigenic changes in Sindbis virus E1 glycoprotein.
    Virology. 1983 Oct 15;130(1):144-54 PMID: 6195815
  27. Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7155-9 PMID: 6580632
  28. Infectious poxvirus vectors have capacity for at least 25 000 base pairs of foreign DNA.
    Gene. 1983 Nov;25(1):21-8 PMID: 6229451
  29. Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):193-7 PMID: 6320164
  30. General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.
    J Virol. 1984 Mar;49(3):857-64 PMID: 6321770
  31. Complete nucleotide sequence of the genomic RNA of Sindbis virus.
    Virology. 1984 Feb;133(1):92-110 PMID: 6322438
  32. Plasmodium knowlesi sporozoite antigen: expression by infectious recombinant vaccinia virus.
    Science. 1984 Apr 27;224(4647):397-9 PMID: 6200932
  33. Envelope proteins of Semliki Forest virus synthesized in Xenopus oocytes are transported to the cell surface.
    EMBO J. 1984 Apr;3(4):767-71 PMID: 6373249
  34. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
    Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
  35. Live recombinant vaccinia virus protects chimpanzees against hepatitis B.
    Nature. 1984 Sep 6-11;311(5981):67-9 PMID: 6472464
  36. Expression of rabies virus glycoprotein from a recombinant vaccinia virus.
    Nature. 1984 Nov 8-14;312(5990):163-6 PMID: 6548799
  37. Detection of immunologically cross-reacting capsid protein of alphaviruses on the surfaces of infected L929 cells.
    J Virol. 1985 Jan;53(1):198-204 PMID: 3965743
  38. Vaccinia virus recombinants: expression of VSV genes and protective immunization of mice and cattle.
    Science. 1985 Jan 25;227(4685):433-5 PMID: 2981435
  39. Sequence analysis of three Sindbis virus mutants temperature-sensitive in the capsid protein autoprotease.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4648-52 PMID: 3895223
  40. Biochemical studies of the maturation of the small Sindbis virus glycoprotein E3.
    Virology. 1984 Apr 30;134(2):338-57 PMID: 6443592
  41. Genetic assay for small fragments of bacteriophage phi X174 deoxyribonucleic acid.
    J Virol. 1971 Aug;8(2):181-9 PMID: 4940243
  42. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  43. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  44. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  45. Cleavage of a viral envelope precursor during the morphogenesis of Sindbis virus.
    J Virol. 1974 Apr;13(4):809-17 PMID: 4821491
  46. Effect of ionic strength on the binding of Sindbis virus to chick cells.
    J Virol. 1974 May;13(5):1030-6 PMID: 4363248
  47. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  48. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  49. Translation of Sindbis virus 26 S RNA and 49 S RNA in lysates of rabbit reticulocytes.
    J Mol Biol. 1974 Jun 25;86(2):397-409 PMID: 4416702
  50. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  51. Encephalomyocarditis virus RNA: variations in polyadenylic acid content and biological activity.
    J Virol. 1976 Aug;19(2):325-30 PMID: 183008
  52. Mutants of sindbis virus. I. Isolation and partial characterization of 89 new temperature-sensitive mutants.
    Virology. 1976 Oct 1;74(1):154-68 PMID: 62444
  53. Hybridization in situ of SV40 plaques: detection of recombinant SV40 virus carrying specific sequences of nonviral DNA.
    Science. 1977 Apr 8;196(4286):183-5 PMID: 191907
  54. Virus-directed post-translational cleavage in Sindbus virus-infected cells.
    J Virol. 1977 May;22(2):568-71 PMID: 559105
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.56.1.227-239.1985
Published
1985-10-00
Pages
227-39
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252510
Subset
IM
Grants
NCRR NIH HHS · 557 RR 07003 · United States
NIAID NIH HHS · AI 10793 · United States
NIAID NIH HHS · AI 20612 · United States
External Links
PubMed source DOI: 10.1128/JVI.56.1.227-239.1985 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2