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PMID: 4010 Published · ppublish English Journal Article

Plasmid-mediated penicillin beta-lactamases in Pseudomonas aeruginosa.

Antimicrobial agents and chemotherapy ·Vol. 9 ·No. 1 ·1976-01-00 ·Pages 55-60

Sawada Y, Yaginuma S, Tai M, Iyobe S, Mitsuhashi S

Abstract

A penicillin beta-lactamase (PCase) was extracted from Pseudomonas aeruginosa Rms139(+) and purified by means of column chromatography. The isoelectric point of Rms139 PCase was 5.7 and its molecular weight was 22,500 +/- 1,000. The optimal pH for the hydrolysis of benzylpenicillin was 7.0 to 7.5 and the optimal temperature was 45 C, with the PCase also showing high activity against carbenicillin. It is concluded that this enzyme is a new type of penicillin beta-lactamase different from the type I, II, or III R plasmid-mediated PCases reported previously.

MeSH Terms
Bacterial Proteins/analysis Conjugation, Genetic Culture Media Drug Resistance, Microbial Extrachromosomal Inheritance Hydrogen-Ion Concentration Molecular Weight Penicillinase/metabolism Plasmids Pseudomonas aeruginosa/drug effects,enzymology R Factors Temperature
Chemicals
Bacterial Proteins Culture Media Penicillinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sawada Y
Yaginuma S
Tai M
Iyobe S
Mitsuhashi S
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.9.1.55
Published
1976-01-00
Pages
55-60
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC429473
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.9.1.55 PMC Full Text View journal details
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