The purification and properties of the -lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilis.

The Biochemical journal ·Vol. 123 ·No. 4 ·1971-07-00 ·Pages 493-500

Dale JW, Smith JT

Abstract

1. The beta-lactamase specified by the R-1818 resistance factor in Escherichia coli was purified 300-fold; the resulting preparation gave a single peak on Sephadex G-100 and a single band on polyacrylamide-gel electrophoresis. 2. The beta-lactamase specified by the same R-factor in Proteus mirabilis was purified over 2000-fold, but was still far from pure. The specific activity of this preparation was one-fifth that of the purified enzyme from E. coli. 3. The two enzymes were shown to be identical as regards substrate specificity, pH optimum, K(m) values and molecular weight. 4. It is suggested that the low beta-lactamase activity of extracts of P. mirabilis (R-1818), about 5% of that from E. coli (R-1818) in crude extracts, could be due to inefficient transcription of the R-factor DNA by Proteus RNA polymerase.

MeSH Terms

Chromatography, Gel DNA, Bacterial Electrophoresis, Disc Escherichia coli/enzymology Genetic Code Hydrogen-Ion Concentration Kinetics Molecular Weight Penicillin Resistance Penicillinase/isolation & purification,metabolism Proteus/enzymology RNA Nucleotidyltransferases

Chemicals

DNA, Bacterial RNA Nucleotidyltransferases Penicillinase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dale J W

Smith J T

References (27)

27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1230493

Published

1971-07-00

Pages

493-500

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1176988

Subset

External Links

PubMed source DOI: 10.1042/bj1230493 PMC Full Text View journal details

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The purification and properties of the -lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilis.

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