Characterization of the -lactamase specified by the resistance factor R-1818 in E. coli K12 and other Gram-negative bacteria.

The Biochemical journal ·Vol. 123 ·No. 4 ·1971-07-00 ·Pages 501-5

Dale JW

Abstract

1. The amino acid composition of the beta-lactamase from E. coli (R-1818) was determined. 2. The R-1818 beta-lactamase is inhibited by formaldehyde, hydroxylamine, sodium azide, iodoacetamide, iodine and sodium chloride. 3. The K(m) values for benzylpenicillin, ampicillin and oxacillin have been determined by using the R-factor enzyme from different host species. The same values were obtained, irrespective of the host bacterium. 4. The molecular weight of the enzyme was found to be 44600, and was the same for all host species. 5. The relationship of R-1818 and R-GN238 beta-lactamases is discussed.

MeSH Terms

Amino Acids/analysis Ampicillin/metabolism Azides/pharmacology Chromatography, Gel Enterobacteriaceae/enzymology Escherichia coli/enzymology Formaldehyde/pharmacology Hydroxylamines/pharmacology Iodine/pharmacology Iodoacetates/pharmacology Kinetics Klebsiella/enzymology Molecular Weight Oxacillin/metabolism Penicillin G/metabolism Penicillin Resistance Penicillinase/analysis,metabolism Proteus/enzymology Serratia marcescens/enzymology Sodium Chloride/pharmacology beta-Lactamase Inhibitors

Chemicals

Amino Acids Azides Hydroxylamines Iodoacetates beta-Lactamase Inhibitors Formaldehyde Sodium Chloride Ampicillin Iodine Penicillinase Penicillin G Oxacillin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Dale J W

References (18)

18 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1230501

Published

1971-07-00

Pages

501-5

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1176989

Subset

External Links

PubMed source DOI: 10.1042/bj1230501 PMC Full Text View journal details

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Characterization of the -lactamase specified by the resistance factor R-1818 in E. coli K12 and other Gram-negative bacteria.

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