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PMID: 4942448 Published · ppublish English Journal Article

Some relationships between R-factor and chromosomal -lactamase in Gram-negative bacteria.

The Biochemical journal ·Vol. 123 ·No. 4 ·1971-07-00 ·Pages 507-12

Dale JW, Smith JT

Abstract

1. The beta-lactamases specified by Klebsiella aerogenes 418 and the R-factor R-7268 have been partially purified. 2. The molecular weights of the K. aerogenes strains 418 and 373, Aerobacter cloacae 53, R-7268 and R-TEM beta-lactamases were all about 20000; that of the enzymes from Escherichia coli strains 419 and 214T was about 31000. 3. These enzymes were also compared by means of their K(m) values for benzylpenicillin and ampicillin, and their behaviour on starch-gel electrophoresis. 4. The beta-lactamases specified by the two Klebsiella strains, the Aerobacter strain, and the R-factors R-TEM and R-7268 were found to comprise a broadly similar group. However, within this group, only two enzymes seemed to be identical, namely those specified by the two R-factors. The two E. coli strains produce identical beta-lactamases which are very different from the ;Klebsiella/Aerobacter-type' enzymes.

MeSH Terms
Ampicillin/metabolism Bacteria/enzymology Electrophoresis Enterobacter/enzymology Escherichia coli/enzymology Isoenzymes/isolation & purification Kinetics Klebsiella/enzymology Molecular Weight Penicillin G/metabolism Penicillin Resistance Penicillinase/isolation & purification,metabolism
Chemicals
Isoenzymes Ampicillin Penicillinase Penicillin G
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dale J W
Smith J T
References (32)
32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1230507
Published
1971-07-00
Pages
507-12
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1176990
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1230507 PMC Full Text View journal details
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