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PMID: 3857618 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid-induced "hit-and-run" tumorigenesis in Chinese hamster embryo fibroblast (CHEF) cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 9 ·1985-05-00 ·Pages 2839-43

Lau CC, Gadi IK, Kalvonjian S, Anisowicz A, Sager R

Abstract

The Chinese hamster embryo fibroblast cell line CHEF/18 is readily transfected by plasmid DNA. In the present transfection studies with CHEF/18 cells, focus formation induced by plasmids containing the mutant human c-Ha-ras gene EJ was compared with that of control plasmids without the EJ insert. The focus-forming activity of the transfected plasmid J132, a recombinant of the Harvey murine sarcoma virus LTR and the normal human c-Ha-ras1 in pBR322, also was assessed. Foci were recovered after transfection with either pSV2gpt or pSV2neo at about 10% the frequency obtained with the EJ-containing plasmids, and J132 gave a similar frequency, all well above background obtained with salmon sperm DNA. Whereas foci from transfection with EJ-containing plasmids contained the EJ DNA, no plasmid DNA was detected in either tumorigenic or tumor-derived cells from foci transfected with pSVgpt, pSVneo, or J132. Evidence that genomic changes were induced by plasmid transfection is based on finding chromosomal aberrations in all expanded foci and tumor-derived cells examined. The results suggest the occurrence of "hit-and-run" tumorigenesis induced by transient plasmid transfection.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Chromosome Aberrations Cricetinae Cricetulus DNA, Neoplasm/genetics Embryo, Mammalian Fibroblasts Harvey murine sarcoma virus/genetics Humans Mice Mice, Nude Neoplasms, Experimental/etiology Plasmids Transfection
Chemicals
DNA, Neoplasm
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lau C C
Gadi I K
Kalvonjian S
Anisowicz A
Sager R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.9.2839
Published
1985-05-00
Pages
2839-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397661
Subset
IM
Grants
NCI NIH HHS · CA09361 · United States
NCI NIH HHS · CA24828 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.9.2839 PMC Full Text View journal details
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