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PMID: 3855535 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple polymorphic alpha- and beta-tubulin mRNAs are present in sea urchin eggs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 1 ·1985-01-00 ·Pages 134-8

Alexandraki D, Ruderman JV

Abstract

Multiple alpha- and beta-tubulin RNAs were found in the mature unfertilized eggs of the sea urchin Lytechinus pictus. The alpha-tubulin RNAs were polymorphic in number, size, and relative amounts in the eggs of different females. Five to seven different size classes [1.75-4.2 kilobases (kb)] were detected on RNA gel blots. All egg preparations contained variable amounts of 1.8- and 2.25-kb beta-tubulin RNAs, and a few of them contained an additional 2.9-kb beta-tubulin RNA. The total amount of alpha-tubulin RNA did not always parallel that of beta-tubulin RNA. A portion of all of the various alpha- and beta-tubulin RNAs were polyadenylylated. RNase H digestions ruled out the possibility that some of these RNAs represented a single transcript bearing different lengths of 3' poly(A). One class of alpha-tubulin RNAs (2.4-2.65 kb) was reduced to 2 kb by RNase H, suggesting the presence of internal oligo(A) regions. All of the egg beta-tubulin RNAs sedimented as free ribonucleoprotein particles. Only a small portion of the 1.75- to 3.6-kb alpha-tubulin RNAs, but most of the 4.2-kb alpha-tubulin RNA, were found on polysomes before fertilization. In the 30-min embryo, small amounts of each of the various alpha- and beta-tubulin RNAs were recruited onto polysomes. Thus, each of the multiple polymorphic alpha- and beta-tubulin RNAs in the egg represent translationally competent mRNA.

MeSH Terms
Animals Female Gene Expression Regulation Molecular Weight Ovum/physiology Poly A/metabolism Polymorphism, Genetic Protein Biosynthesis RNA, Messenger/genetics Sea Urchins/embryology,genetics Tubulin/genetics
Chemicals
RNA, Messenger Tubulin Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Alexandraki D
Ruderman J V
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.1.134
Published
1985-01-00
Pages
134-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC396986
Subset
IM
Grants
NICHD NIH HHS · HD15351 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.1.134 PMC Full Text View journal details
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