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PMID: 569291 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA polymerase alpha and beta in the California urchin.

Nucleic acids research ·Vol. 5 ·No. 10 ·1978-10-00 ·Pages 3945-57

Racine FM, Morris PW

Abstract

DNA polymerase alpha and beta were identified in the urchin, Strongylocentrotus purpuratus. The DNA polymerase beta sedimented at 3.4 S, constituted 5% of total DNA polymerase activity, and was resistant to N-ethylmaleimide and high ionic strength. The polymerase alpha sedimented at 6--8 S, was inhibited by N-ethylmalemide or 0.1 M (NH4)2SO4, and was dependent upon glycerol for preservation of activity. Both the polymerases alpha and beta were nuclear associated in embryos. The DNA polymerase alpha was markedly heterogeneous on DEAE-Sephadex ion exchange and showed three modal polymerase species. These polymerase alpha species were indistinguishable by template activity assays but the DNA polymerase associated ribonucleotidyl transferase (Biochemistry 75 : 3106-3113, 1976) was found predominantly with only one of the DNA polymerase alpha species.

MeSH Terms
Animals Cell Nucleus/enzymology DNA Replication/drug effects DNA-Directed DNA Polymerase/isolation & purification,metabolism Female Glycerol/pharmacology Molecular Weight Ovum/enzymology Sea Urchins/embryology,metabolism Substrate Specificity Templates, Genetic
Chemicals
DNA-Directed DNA Polymerase Glycerol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Racine F M
Morris P W
References (26)
26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.10.3945
Published
1978-10-00
Pages
3945-57
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342721
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.10.3945 PMC Full Text View journal details
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