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PMID: 6199660 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Saccharomyces cerevisiae killer virus transcripts contain template-coded polyadenylate tracts.

Molecular and cellular biology ·Vol. 4 ·No. 1 ·1984-01-00 ·Pages 101-9

Hannig EM, Thiele DJ, Leibowitz MJ

Abstract

The M double-stranded RNA component of type 1 killer strains of the yeast Saccharomyces cerevisiae contains an internal 200-base pair adenine- and uracil-rich region. The plus strands of this viral genomic RNA contain an internal adenine-rich region which allows these strands to bind to polyuridylate-Sepharose as tightly as do polyadenylated RNAs with 3'-terminal polyadenylated tracts of 70 to 100 residues. Internal template coding of an adenine-rich tract in positive polarity in vivo and in vitro transcripts of M double-stranded RNA may serve as an alternate method of transcript polyadenylation. The 3'-terminal residue of the in vitro m transcript is a non-template-encoded purine residue. The 5' terminus of this transcript is involved in a stem-and-loop structure which includes an AUG initiation codon, along with potential 18S and 5.8S rRNA binding sites. Except for the 3'-terminal residue, transcription in in vitro shows complete fidelity.

MeSH Terms
Base Sequence Nucleic Acid Conformation Poly A/metabolism RNA/metabolism RNA Viruses/metabolism RNA, Double-Stranded/metabolism RNA, Messenger RNA, Viral/metabolism Saccharomyces cerevisiae/metabolism Transcription, Genetic
Chemicals
RNA, Double-Stranded RNA, Messenger RNA, Viral Poly A RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hannig E M
Thiele D J
Leibowitz M J
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51 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.1.101-109.1984
Published
1984-01-00
Pages
101-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368663
Subset
IM
Grants
NIAID NIH HHS · AI-14843 · United States
NCI NIH HHS · CA-09069 · United States
Databases
GENBANK
M12522
External Links
PubMed source DOI: 10.1128/mcb.4.1.101-109.1984 PMC Full Text View journal details
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