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PMID: 6287219 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence heterogeneity, multiplicity, and genomic organization of alpha- and beta-tubulin genes in sea urchins.

Molecular and cellular biology ·Vol. 1 ·No. 12 ·1981-12-00 ·Pages 1125-37

Alexandraki D, Ruderman JV

Abstract

We analyzed the multiplicity, heterogeneity, and organization of the genes encoding the alpha and beta tubulins in the sea urchin Lytechinus pictus by using cloned complementary deoxyribonucleic acid (cDNA) and genomic tubulin sequences. cDNA clones were constructed by using immature spermatogenic testis polyadenylic acid-containing ribonucleic acid as a template. alpha- and beta-tubulin clones were identified by hybrid selection and in vitro translation of the corresponding messenger ribonucleic acids, followed by immunoprecipitation and two-dimensional gel electrophoresis of the translation products. The alpha cDNA clone contains a sequence that encodes the 48 C-terminal amino acids of alpha tubulin and 104 base pairs of the 3' nontranslated portion of the messenger ribonucleic acid. The beta cDNA insertion contains the coding sequence for the 100-C terminal amino acids of beta tubulin and 83 pairs of the 3' noncoding sequence. Hybrid selections performed at different criteria demonstrated the presence of several heterogeneous, closely related tubulin messenger ribonucleic acids, suggesting the existence of heterogeneous alpha- and beta-tubulin genes. Hybridization analyses indicated that there are at least 9 to 13 sequences for each of the two tubulin gene families per haploid genome. Hybridization of the cDNA probes to both total genomic DNA and cloned germline DNA fragments gave no evidence for close physical linkage of alpha-tubulin genes with beta-tubulin genes at the DNA level. In contrast, these experiments indicated that some genes within the same family are clustered.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Gene Expression Regulation Genes Nucleic Acid Hybridization Protein Biosynthesis Sea Urchins/embryology,genetics Tubulin/genetics
Chemicals
DNA, Recombinant Tubulin DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Alexandraki D
Ruderman J V
References (50)
50 references, click to expand
  1. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  2. A dispersed multigene family encoding tubulin in Drosophila melanogaster.
    Cell. 1980 Dec;22(3):845-54 PMID: 6780200
  3. Biosynthesis of heterogeneous forms of mammalian brain tubulin subunits by multiple messenger RNAs.
    J Neurochem. 1979 Jul;33(1):231-46 PMID: 458452
  4. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  6. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  7. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  8. Chlamydomonas flagella. II. The distribution of tubulins 1 and 2 in the outer doublet microtubules.
    J Cell Biol. 1972 Sep;54(3):540-55 PMID: 5044758
  9. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  10. Nucleotide and corresponding amino acid sequences encoded by alpha and beta tubulin mRNAs.
    Nature. 1981 Feb 19;289(5799):650-5 PMID: 7464932
  11. Multiple alpha and beta tubulin genes represent unlinked and dispersed gene families.
    J Biol Chem. 1981 Mar 25;256(6):3130-4 PMID: 6259149
  12. The nucleotide sequence of the lac operator.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4 PMID: 4587255
  13. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  14. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
  15. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
    Cell. 1974 Dec;3(4):315-25 PMID: 4216403
  16. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  17. Programmed synthesis of flagellar tubulin during cell differentiation in Naegleria.
    Ann N Y Acad Sci. 1975 Jun 30;253:318-32 PMID: 1056749
  18. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  19. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  20. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  21. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  22. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
    Nucleic Acids Res. 1976 Jan;3(1):101-16 PMID: 765970
  23. Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.
    J Cell Biol. 1976 May;69(2):301-12 PMID: 1262392
  24. Enzymatic in vitro synthesis of globin genes.
    Cell. 1976 Feb;7(2):279-88 PMID: 60178
  25. Biochemistry and physiology of microtubules.
    Annu Rev Biochem. 1976;45:699-720 PMID: 786160
  26. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  27. Microtubules: structure, chemistry, and function.
    Physiol Rev. 1976 Oct;56(4):709-77 PMID: 790425
  28. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent.
    J Immunol. 1976 Nov;117(5 Pt 1):1482-90 PMID: 187693
  29. Tubulin synthesis in sea urchin embryos: almost all tubulin of the first cleavage mitotic apparatus derives from the unfertilized egg.
    Dev Biol. 1977 Jan;55(1):191-5 PMID: 556707
  30. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  31. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  32. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  33. Major membrane protein differences in cilia and flagella: evidence for a membrane-associated tubulin.
    Biochemistry. 1977 May 17;16(10):2047-58 PMID: 861196
  34. Comparison of the isoelectric and molecular weight properties of tubulin subunits.
    J Neurochem. 1977 Apr;28(4):697-706 PMID: 894278
  35. Differential protein synthesis and utilization during cilia formation in sea urchin embryos.
    Dev Biol. 1977 Dec;61(2):311-29 PMID: 145385
  36. Separate mRNAs code for tubulin subunits.
    Nature. 1978 Mar 2;272(5648):81-3 PMID: 628438
  37. A portion of all major classes of histone messenger RNA in amphibian oocytes is polyadenylated.
    J Biol Chem. 1978 Mar 25;253(6):2018-25 PMID: 564902
  38. Simultaneous localization of myosin and tubulin in human tissue culture cells by double antibody staining.
    J Cell Biol. 1978 Apr;77(1):182-95 PMID: 350890
  39. Primary structural differences among tubulin subunits from flagella, cilia, and the cytoplasm.
    Biochemistry. 1978 Jul 11;17(14):2882-91 PMID: 567486
  40. A novel arrangement of tandemly repeated genes at a major heat shock site in D. melanogaster.
    Cell. 1978 Aug;14(4):901-19 PMID: 99245
  41. A bacterial clone synthesizing proinsulin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3727-31 PMID: 358198
  42. Identification of a gene for beta-tubulin in Aspergillus nidulans.
    Cell. 1978 Oct;15(2):639-47 PMID: 363278
  43. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  44. Mutation in a structural gene for a beta-tubulin specific to testis in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3991-5 PMID: 115008
  45. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  46. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  47. Identification of chorion protein precursors and the mRNAs that encode them in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6279-83 PMID: 118465
  48. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
    Cell. 1980 May;20(1):95-105 PMID: 6893015
  49. Mutation in a testis-specific beta-tubulin in Drosophila: analysis of its effects on meiosis and map location of the gene.
    Cell. 1980 Sep;21(2):445-51 PMID: 6773669
  50. Isolation of separate mRNAs for alpha- and beta-tubulin and characterization of the corresponding in vitro translation products.
    Cell. 1978 Nov;15(3):1021-31 PMID: 728983
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.12.1125-1137.1981
Published
1981-12-00
Pages
1125-37
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369739
Subset
IM
Grants
NICHD NIH HHS · HD 00349 · United States
NICHD NIH HHS · HD 10994 · United States
External Links
PubMed source DOI: 10.1128/mcb.1.12.1125-1137.1981 PMC Full Text View journal details
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