Home LiteratureArticle Details
PMID: 378953 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase.

Journal of bacteriology ·Vol. 139 ·No. 1 ·1979-07-00 ·Pages 165-75

Baer M, Low KB, Söll D

Abstract

Spontaneous revertants of a temperature-sensitive Escherichia coli strain harboring a thermolabile valyl-transfer ribonucleic acid (tRNA) synthetase were selected for growth at 40 degrees C. Of these, a large number still contain the thermolabile valyl-tRNA synthetase. Three of these revertants contained an increased level of the thermolabile enzyme. The genetic locus, valX, responsible for the enzyme overproduction, is adjacent to the structural gene, valS, of valyl-tRNA synthetase. Determination (by radioimmunoassay) of the turnover rates of valyl-tRNA synthetase showed that the increased level of valyl-tRNA synthetase is due to new enzyme synthesis rather than decreased rates of protein degradation.

MeSH Terms
Amino Acyl-tRNA Synthetases/biosynthesis,metabolism Bacterial Proteins/biosynthesis Escherichia coli/enzymology,genetics Genes, Regulator Mutation RNA, Bacterial/metabolism RNA, Transfer/metabolism Valine-tRNA Ligase/biosynthesis,genetics
Chemicals
Bacterial Proteins RNA, Bacterial RNA, Transfer Amino Acyl-tRNA Synthetases Valine-tRNA Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baer M
Low K B
Söll D
References (32)
32 references, click to expand
  1. Genetic variation in the sex factor of Escherichia coli.
    J Bacteriol. 1960 Mar;79:321-30 PMID: 13791860
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. VI. Mutants with increased levels of glutaminyl-transfer ribonucleic acid synthetase and of glutamine transfer ribonucleic acid.
    J Bacteriol. 1979 Jul;139(1):176-84 PMID: 378954
  4. Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase.
    Mol Gen Genet. 1979 Jan 31;169(2):205-11 PMID: 375009
  5. Regulation of the biosynthesis of aminoacid: tRNA ligases and of tRNA.
    Prog Nucleic Acid Res Mol Biol. 1978;21:181-207 PMID: 358278
  6. Transient rates of synthesis of individual polypeptides in E. coli following temperature shifts.
    Cell. 1978 Mar;13(3):427-34 PMID: 350413
  7. Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity.
    Mol Gen Genet. 1977 Nov 14;156(2):221-7 PMID: 340903
  8. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. II. Isolation of regulatory mutants affecting leucyl-tRNA synthetase levels.
    J Mol Biol. 1977 Dec 25;117(4):1033-48 PMID: 342703
  9. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. I. Isolation and characterization of a mutant with elevated levels of tRNAGln 1.
    J Mol Biol. 1977 Dec 25;117(4):1013-31 PMID: 24122
  10. Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in Escherichia coli.
    J Bacteriol. 1977 Jan;129(1):378-87 PMID: 318645
  11. Regulation of amino acid transport in Escherichia coli by transcription termination factor rho.
    J Bacteriol. 1977 Jun;130(3):1024-9 PMID: 324970
  12. Regulation of synthesis of the branched-chain amino acids and cognate aminoacyl-transfer ribonucleic acid synthetases of Escherichia coli: a common regulatory element.
    J Bacteriol. 1974 Dec;120(3):1380-6 PMID: 4612020
  13. Alteration of structure of level of threonyl-tRNA-synthetase in Borrelidin resistant mutants of E. coli.
    FEBS Lett. 1974 Feb 15;39(2):182-6 PMID: 4368395
  14. Metabolic regulation of the arginyl and valyl transfer ribonucleic acid synthetases in bacteria.
    J Biol Chem. 1974 Feb 25;249(4):1044-53 PMID: 4592258
  15. Thiosine-resistant mutants of Escherichia coli K-12 with growth-medium-dependent lysl-tRNA synthetase activity. I. Isolation and physiological characterization.
    Biochim Biophys Acta. 1972 Feb 15;259(3):330-43 PMID: 4552089
  16. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.
    J Bacteriol. 1971 Nov;108(2):742-50 PMID: 4942762
  17. Metabolic regulation of aminoacyl-tRNA synthetase formation in bacteria.
    Biochem Biophys Res Commun. 1972 Oct 17;49(2):495-501 PMID: 4565494
  18. Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12.
    J Bacteriol. 1973 Mar;113(3):1091-5 PMID: 4570768
  19. Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12.
    Eur J Biochem. 1973 Jun;35(2):331-7 PMID: 4577856
  20. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference.
    Anal Biochem. 1968 Feb;22(2):195-210 PMID: 4171014
  21. Solid phase radioimmunoassay of human beta 1C globulin.
    Immunochemistry. 1970 Jan;7(1):29-41 PMID: 4190345
  22. Growth rate modulation of four aminoacyl-transfer ribonucleic acid synthetases in enteric bacteria.
    J Bacteriol. 1976 May;126(2):634-45 PMID: 177401
  23. Function and regulation of aminoacyl-tRNA synthetases in prokaryotic and eukaryotic cells.
    Annu Rev Microbiol. 1975;29:215-50 PMID: 1180514
  24. Enhanced level and metabolic regulation of methionyl-transfer ribonucleic acid synthetase in different strains of Escherichia coli K-12.
    J Bacteriol. 1975 Aug;123(2):580-8 PMID: 1097418
  25. Characterization of a mutant of E. coli with elevated levels of seryl-tRNA synthetase.
    Biochem Biophys Res Commun. 1972 Dec 4;49(5):1351-7 PMID: 4566679
  26. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.
    J Bacteriol. 1973 Feb;113(2):798-812 PMID: 4570607
  27. Degradation of abnormal proteins in Escherichia coli (protein breakdown-protein structure-mistranslation-amino acid analogs-puromycin).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):422-6 PMID: 4551144
  28. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.
    Biochem J. 1973 Jul;133(3):529-39 PMID: 4733239
  29. In vivo degradation of mutant lac repressor.
    Nature. 1970 Dec 19;228(5277):1154-6 PMID: 4922498
  30. The control of ribonucleic acid synthesis in Escherichia coli. IV. Relevance of unusual phosphorylated compounds from amino acid-starved stringent strains.
    J Biol Chem. 1969 Jun 25;244(12):3133-41 PMID: 4893338
  31. Missense mutations in the lacZ gene that result in degradation of beta-galactosidase structural protein.
    J Bacteriol. 1976 Sep;127(3):1538-42 PMID: 783147
  32. Effect of the RelA gene on the synthesis of individual proteins in vivo.
    Cell. 1976 May;8(1):115-22 PMID: 782722
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.139.1.165-175.1979
Published
1979-07-00
Pages
165-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216842
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.139.1.165-175.1979 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2