Regulation of synthesis of the branched-chain amino acids and cognate aminoacyl-transfer ribonucleic acid synthetases of Escherichia coli: a common regulatory element.

Journal of bacteriology ·Vol. 120 ·No. 3 ·1974-12-00 ·Pages 1380-6

Jackson J, Williams LS, Umbarger HE

Abstract

Regulation of isoleucine, valine, and leucine biosynthesis and isoleucyl-, valyl-, and leucyl-transfer ribonucleic acid (tRNA) synthetase formation was examined in two mutant strains of Escherichia coli. One mutant was selected for growth resistance to the isoleucine analogue, ketomycin, and the other was selected for growth resistance to both trifluoroleucine and valine. Control of the synthesis of the branched-chain amino acids by repression was altered in both of these mutants. They also exhibited altered control of formation of isoleucyl-tRNA synthetase (EC 6.1.15, isoleucine:sRNA ligase, AMP), valyl-tRNA synthetase (EC 6.1.1.9, valine:sRNA ligase, AMP), and leucyl-tRNA synthetase (EC 6.1.1.4, leucine:sRNA ligase, AMP). These results suggest the existence of a common element for the control of these two classes of enzymes in Escherichia coli.

MeSH Terms

Alcohol Oxidoreductases/metabolism Amino Acids/metabolism Amino Acyl-tRNA Synthetases/biosynthesis,metabolism Carbon Radioisotopes Cell-Free System Enzyme Repression Escherichia coli/enzymology,growth & development,metabolism Fluorine Genes, Regulator Hydro-Lyases/metabolism Isoleucine/analogs & derivatives,biosynthesis Leucine/analogs & derivatives,biosynthesis Ligases/metabolism Mutation Threonine Valine/biosynthesis,pharmacology

Chemicals

Amino Acids Carbon Radioisotopes Isoleucine Fluorine Threonine Alcohol Oxidoreductases Hydro-Lyases Ligases Amino Acyl-tRNA Synthetases Leucine Valine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jackson J

Williams L S

Umbarger H E

References (11)

11 references, click to expand

Histidine regulatory mutants in Salmonella typhimurium 3. A class of regulatory mutants deficient in tRNA for histidine.
J Mol Biol. 1966 Dec 28;22(2):335-47 PMID: 5339689
Modification of valyl tRNA synthetase by bacteriophage in Escherichia coli.
J Mol Biol. 1968 Feb 14;31(3):463-75 PMID: 4866334
Isoleucine and valine metabolism of Escherichia coli. XIV. Effect of thiaisoleucine.
J Bacteriol. 1968 May;95(5):1666-71 PMID: 4870281
Purification and properties of beta-isopropylmalate dehydrogenase.
J Biol Chem. 1969 Feb 10;244(3):996-1003 PMID: 4889950
Mutants of Salmonella typhimurium with an altered leucyl-transfer ribonucleic acid synthetase.
J Bacteriol. 1971 Apr;106(1):213-20 PMID: 4928008
Regulation of synthesis of the aminoacyl-transfer ribonucleic acid synthetases for the branched-chain amino acids of Escherichia coli.
J Bacteriol. 1971 Oct;108(1):254-62 PMID: 4941558
On the role of isoleucyl-tRNA synthetase in multivalent repression.
Biochem Genet. 1972 Apr;6(2):99-118 PMID: 4581142
Defective transamination, a mechanism for resistance to ketomycin in Escherichia coli.
Antimicrob Agents Chemother. 1973 Apr;3(4):510-6 PMID: 4597727
Use of chloramphenicol to study control of RNA synthesis in bacteria.
Biochim Biophys Acta. 1961 Oct 14;53:96-110 PMID: 13894423
Mutants of Escherichia coli requiring methionine or vitamin B12.
J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
ROLE OF VALYL-SRNA SYNTHETASE IN ENZYME REPRESSION.
Proc Natl Acad Sci U S A. 1965 Mar;53:539-43 PMID: 14338232

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.120.3.1380-1386.1974

Published

1974-12-00

Pages

1380-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245925

Subset

External Links

PubMed source DOI: 10.1128/jb.120.3.1380-1386.1974 PMC Full Text View journal details

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Regulation of synthesis of the branched-chain amino acids and cognate aminoacyl-transfer ribonucleic acid synthetases of Escherichia coli: a common regulatory element.

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