Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase.

Molecular & general genetics : MGG ·Vol. 169 ·No. 2 ·1979-01-31 ·Pages 205-11

Theall G, Low KB, Söll D

Abstract

Spontaneous revertants of a temperature-sensitive Escherichia coli strain harboring a thermolabile leucyl-tRNA synthetase and seryl-tRNA synthetase were selected for growth at 40 degrees C. Among these, strains were found with increased levels of both thermolabile synthetases. Two distinct genetic loci were found responsible for enzyme overproduction. leuR, located near xyl, causes elevated levels of leucyl-tRNA synthetase; while serR, located near leu, causes elevated levels of seryl-tRNA synthetase.

MeSH Terms

Amino Acyl-tRNA Synthetases/genetics Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics,metabolism Genes, Regulator Leucine-tRNA Ligase/biosynthesis,genetics Mutation RNA, Bacterial/biosynthesis,genetics RNA, Transfer/biosynthesis,genetics Serine-tRNA Ligase/genetics Temperature

Chemicals

RNA, Bacterial RNA, Transfer Amino Acyl-tRNA Synthetases Serine-tRNA Ligase Leucine-tRNA Ligase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Theall G

Low K B

Söll D

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00271672

Published

1979-01-31

Pages

205-11

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00271672

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Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase.

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