Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.

Journal of bacteriology ·Vol. 108 ·No. 2 ·1971-11-00 ·Pages 742-50

Low B, Gates F, Goldstein T, Söll D

Abstract

Two temperature-sensitive mutants of Escherichia coli have been found in which the conditional growth is a result of a thermosensitive leucyl-transfer ribonucleic acid (tRNA) synthetase and seryl-tRNA synthetase, respectively. The corresponding genetic loci, leuS and serS, cotransduce with lip and serC, respectively. As a result of the mutationally altered leucyl-tRNA synthetase, some leucine-, valine-, and isoleucine-forming enzymes were derepressed. Thus, leucyl-tRNA synthetase is involved in the repression of the enzymes needed for the synthesis of branched-chain amino acids.

MeSH Terms

Acylation Amino Acids/metabolism Amino Acyl-tRNA Synthetases/metabolism Carbon Isotopes Cell-Free System Chromosome Mapping Crosses, Genetic Culture Media Enzyme Repression Escherichia coli/enzymology,growth & development,isolation & purification,metabolism Genetics, Microbial Immune Sera Isoleucine/biosynthesis Leucine/biosynthesis Mutagens Mutation Nitrosoguanidines Serine Temperature Transduction, Genetic Valine/biosynthesis

Chemicals

Amino Acids Carbon Isotopes Culture Media Immune Sera Mutagens Nitrosoguanidines Isoleucine Serine Amino Acyl-tRNA Synthetases Leucine Valine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Low B

Gates F

Goldstein T

Söll D

References (35)

35 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.2.742-750.1971

Published

1971-11-00

Pages

742-50

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247134

Subset

External Links

PubMed source DOI: 10.1128/jb.108.2.742-750.1971 PMC Full Text View journal details

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Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.

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