Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.

Journal of bacteriology ·Vol. 95 ·No. 3 ·1968-03-00 ·Pages 991-7

Kaplan S, Anderson D

Abstract

A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.

MeSH Terms

Amino Acids/metabolism Bacterial Proteins/biosynthesis Carbon Isotopes Escherichia coli/enzymology Ligases/metabolism Molecular Biology Mutation Proline/metabolism RNA, Bacterial/biosynthesis Temperature Thymine/metabolism Tritium Uracil/metabolism

Chemicals

Amino Acids Bacterial Proteins Carbon Isotopes RNA, Bacterial Tritium Uracil Proline Ligases Thymine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kaplan S

Anderson D

References (16)

16 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.95.3.991-997.1968

Published

1968-03-00

Pages

991-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC252122

Subset

External Links

PubMed source DOI: 10.1128/jb.95.3.991-997.1968 PMC Full Text View journal details

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Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.

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