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PMID: 3774548 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 7823-38

Sunnerhagen P, Sjöberg RM, Karlsson AL, Lundh L, Bjursell G

Abstract

We have isolated, cloned and analyzed small polydisperse circular (spc) DNA from mouse 3T6 cells. The representation of highly repeated mouse genome sequence families in spcDNA has been examined, and the B1 repeat appears overrepresented in spcDNA by two criteria. The majority of spcDNA clones, however, is made out by as yet uncharacterized middle repetitive sequences. We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.

MeSH Terms
Animals Cell Line Cloning, Molecular Cycloheximide/pharmacology DNA, Circular/genetics,isolation & purification Gene Amplification Mice/genetics Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Circular Cycloheximide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sunnerhagen P
Sjöberg R M
Karlsson A L
Lundh L
Bjursell G
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.20.7823
Published
1986-10-24
Pages
7823-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311817
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.20.7823 PMC Full Text View journal details
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