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PMID: 6700583 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of small circular DNA from Chinese hamster ovary cells.

Molecular and cellular biology ·Vol. 4 ·No. 1 ·1984-01-00 ·Pages 173-80

Stanfield SW, Helinski DR

Abstract

Small polydisperse circular (spc) DNA was isolated and cloned, using BglII from Chinese hamster ovary (CHO) cells. The properties of 47 clones containing at least 43 different BglII fragments are reported. The majority of the clones probably contain entire sequences from individual spcDNA molecules. Most of the clones were homologous to sequences in CHO cell chromosomal DNA, and many were also homologous to mouse LMTK- cell chromosomal sequences. The majority of homologous CHO cell chromosomal sequences were repetitive, although a few may be single copy. Only a small fraction of cloned spcDNA molecules were present in every cell; most occurred less frequently than once in 15 cells. Localization studies indicated that at least a portion of spcDNA is associated with the nucleus in CHO cells.

MeSH Terms

Animals Cells, Cultured Chromosomes Cloning, Molecular Cricetinae Cricetulus DNA, Circular/genetics,isolation & purification Female Mice Ovary Repetitive Sequences, Nucleic Acid

Chemicals

DNA, Circular

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stanfield S W

Helinski D R

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25 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.4.1.173-180.1984

Published

1984-01-00

Pages

173-80

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC368672

Subset

Grants

NIAID NIH HHS · AI-07194 · United States

External Links

PubMed source DOI: 10.1128/mcb.4.1.173-180.1984 PMC Full Text View journal details

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Cloning and characterization of small circular DNA from Chinese hamster ovary cells.

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