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PMID: 375021 Published · ppublish English Journal Article

DNA synthesis in UV-irradiated Escherichia coli K-12 strains carrying dnaA mutations.

Molecular & general genetics : MGG ·Vol. 171 ·No. 1 ·1979-03-09 ·Pages 53-8

Jonczyk P, Cieśla Z

Abstract

It has been found that UV irradiation of dnaA mutants of E. coli K-12 enables the initiation of DNA synthesis at a temperature restrictive to these mutants. The UV-induced DNA synthesis is dependent on protein synthesis and on a transcriptional event at a time when protein synthesis is no longer required. In contrast to dnaA mutants UV irradiation fails to induce DNA synthesis in the two other initiation mutants dnaC2 and dnaB252. DNA synthesis at the restrictive temperature is initiated also when the tif-1 phenotype is expressed in the dnaA46 tif-1 double mutant. Possible mechanisms of the observed capability of dnaA mutants to synthesize DNA at the restrictive temperature after UV irradiation or under conditions of tif-1 expression are discussed.

MeSH Terms
Bacterial Proteins/biosynthesis Chloramphenicol/pharmacology Chromosomes, Bacterial/radiation effects DNA Replication/drug effects DNA, Bacterial/genetics,radiation effects Escherichia coli/genetics,radiation effects Mutation/radiation effects Phenotype Protein Biosynthesis Rifampin/pharmacology Temperature Ultraviolet Rays
Chemicals
Bacterial Proteins DNA, Bacterial Chloramphenicol Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jonczyk P
Cieśla Z
References (21)
21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00274014
Published
1979-03-09
Pages
53-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00274014
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