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PMID: 1107815 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12.

Molecular & general genetics : MGG ·Vol. 141 ·No. 3 ·1975-12-01 ·Pages 189-205

Ganesan AK, Seawell PC

Abstract

We have examined lexA1 uvrA6 and recF143 uvrBdelta derivatives of Escherichia coli K-12 for post-replication repair and DNA synthesis after UV irradiation. Compared to corresponding lex+ rec+ strains, we found that the lexA and recF cells were defective in (1) converting short DNA segments synthesized after irradiation to DNA of normal size; (2) synthesizing high molecular weight DNA after irradiation; (3) transferring pyrimidine dimers from irradiated DNA into unirradiated daughter strands. Our results support the hypothesis that after UV irradiation the formation of large DNA molecules in excision-deficient cells of E.coli depends directly or indirectly upon joining short DNA segments into longer strands, concomitant with the transfer of DNA from irradiated tamplates into unirradiated daughter strands. This process appears to require the activity of lexA and recF genes.

MeSH Terms

DNA Repair/radiation effects DNA, Bacterial/biosynthesis Deoxyribodipyrimidine Photo-Lyase/metabolism Escherichia coli/radiation effects Molecular Weight Mutation Polymers/metabolism Radiation Genetics Ultraviolet Rays

Chemicals

DNA, Bacterial Polymers Deoxyribodipyrimidine Photo-Lyase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ganesan A K

Seawell P C

References (23)

23 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00341799

Published

1975-12-01

Pages

189-205

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00341799

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The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12.

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