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PMID: 164454 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The ultraviolet endonuclease of bacteriophage T4. Further characterization.

The Journal of biological chemistry ·Vol. 250 ·No. 8 ·1975-04-25 ·Pages 2823-9

Minton K, Durphy M, Taylor R, Friedberg EC

Abstract

The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.

MeSH Terms

Animals Cattle Coliphages/enzymology DNA Viruses/enzymology DNA, Single-Stranded/radiation effects DNA, Viral/radiation effects Deoxyribonucleases/metabolism Endonucleases/metabolism Kinetics Molecular Weight Nucleic Acid Denaturation Phosphoric Diester Hydrolases Radiation Effects Spleen/enzymology Thymine/metabolism Time Factors Ultraviolet Rays

Chemicals

DNA, Single-Stranded DNA, Viral Deoxyribonucleases Endonucleases Phosphoric Diester Hydrolases Thymine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Minton K

Durphy M

Taylor R

Friedberg E C

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1975-04-25

Pages

2823-9

Language

English

Region

United States

NLM ID

2985121R

Subset

External Links

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The ultraviolet endonuclease of bacteriophage T4. Further characterization.

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