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PMID: 329277 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 6 ·1977-06-00 ·Pages 2283-7

Roberts JW, Roberts CW, Mount DW

Abstract

We have reproduced in vitro the inactivation of bacteriophage lambda repressor that occurs in vivo when a lambda lysogen is treated with agents such as ultraviolet radiation that attack DNA. ATP and a divalent cation are required for the inactivation reaction. The ind- repressor is insensitive to the inactivation mechanism. A proteolytic cleavage of repressor accompanies inactivation in vitro, as it does in vivo.

MeSH Terms
Adenosine Triphosphate/metabolism Coliphages/physiology Escherichia coli/physiology Genotype Kinetics Lysogeny Mutation Virus Replication
Chemicals
Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roberts J W
Roberts C W
Mount D W
References (16)
16 references, click to expand
  1. [System of repression insuring immunity in lysogenic bacteria].
    C R Hebd Seances Acad Sci. 1959 Jun 1;248(22):3219-21 PMID: 13663329
  2. A method for the isolation of phage mutants altered in their response ot lysogenic induction.
    Mol Gen Genet. 1976 May 7;145(2):165-7 PMID: 934047
  3. A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):300-4 PMID: 319458
  4. Construction of plasmids carrying the cI gene of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 PMID: 1069307
  5. [Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1-mediated induction and filamentation].
    Mol Gen Genet. 1972;119(2):153-74 PMID: 4577433
  6. Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation.
    Mol Gen Genet. 1972;119(2):139-52 PMID: 4565754
  7. Thermal enhancement of ultraviolet mutability in a tif-1 uvrA derivative of Escherichia coli B-r: evidence that ultraviolet mutagenesis depends upon an inducible function.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1930-4 PMID: 4600265
  8. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5 PMID: 4560696
  9. Conditional induction of lambda-prophage in exrA mutants of Escherichia coli.
    Genet Res. 1971 Apr;17(2):161-3 PMID: 4937837
  10. Replication and repair of DNA in cells of Escherichia coli treated with toluene.
    Proc Natl Acad Sci U S A. 1970 Oct;67(2):674-81 PMID: 4943178
  11. Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda.
    J Mol Biol. 1967 Jan 28;23(2):117-33 PMID: 5340183
  12. Intracellular protein degradation in mammalian and bacterial cells: Part 2.
    Annu Rev Biochem. 1976;45:747-803 PMID: 786161
  13. Proteolytic cleavage of bacteriophage lambda repressor in induction.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):147-51 PMID: 1090931
  14. Mechanism of action of Salmonella phage P22 antirepressor.
    J Mol Biol. 1975 Oct 25;98(2):413-24 PMID: 1104871
  15. Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif.
    Mol Gen Genet. 1975 Oct 22;140(4):309-332 PMID: 1107802
  16. A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):54-8 PMID: 264694
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.6.2283
Published
1977-06-00
Pages
2283-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432154
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.6.2283 PMC Full Text View journal details
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