Home LiteratureArticle Details
PMID: 372561 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning.

Journal of virology ·Vol. 29 ·No. 2 ·1979-02-00 ·Pages 555-75

Williams BG, Blattner FR

Abstract

Twenty hybrid lambda phages especially designed for molecular cloning have been constructed and named Charon phages. These phages differ in the ranges of sizes of DNA fragments that may be inserted, by the selections and screens which may be used to isolate and detect the incorporation of cloned fragments, by the way transcription of the cloned fragment may be controlled, by the different restriction enzymes that can be used for cloning, by the phage immunities that may be employed for controlling replication and transcription, and by the biological safety features that they contain. The crosses used to produce the vectors are described, and their genealogy is discussed. The structure of each vector has been verified by genetic tests, by DNA length determinations, by electron micrographic analysis of DNA heteroduplexes, and by gel electrophoresis of restriction enzyme digests. In the course of these constructions, a new EcoRI site was found in a derivative of lambda Aam32Bam1 which maps very near the left cohesive end of lambda.

MeSH Terms
Coliphages/genetics DNA, Bacterial/genetics DNA, Recombinant DNA, Viral/genetics Escherichia coli/genetics Hybridization, Genetic Mutation Phenotype
Chemicals
DNA, Bacterial DNA, Recombinant DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Williams B G
Blattner F R
References (50)
50 references, click to expand
  1. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.
    Genetics. 1954 Jul;39(4):440-52 PMID: 17247495
  2. RECOMBINATION BETWEEN COLIPHAGES LAMBDA AND PHI-80.
    Virology. 1964 Apr;22:650-1 PMID: 14166126
  3. Sensitive mutants of bacteriophage lambda.
    Virology. 1961 May;14:22-32 PMID: 13690195
  4. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization.
    Virology. 1957 Dec;4(3):509-21 PMID: 13507311
  5. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  6. An improved bacteriophage lambda vector: construction of model recombinants coding for kanamycin resistance.
    Gene. 1977 May;1(3-4):209-27 PMID: 338417
  7. Expression of ribosomal protein genes cloned in Charon vector phages and identification of their promoters.
    J Biol Chem. 1977 Oct 25;252(20):7355-64 PMID: 332693
  8. Insertion of DNA carrying ribosomal protein genes of Escherichia coli into Charon vector phages.
    J Biol Chem. 1977 Oct 25;252(20):7344-54 PMID: 332692
  9. Lambdoid phages that simplify the recovery of in vitro recombinants.
    Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
  10. Physical structure of the replication origin of bacteriophage lambda.
    Science. 1977 Dec 9;198(4321):1051-6 PMID: 929187
  11. Genetic structure of the replication origin of bacteriophage lambda.
    Science. 1977 Dec 9;198(4321):1046-51 PMID: 929186
  12. Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda.
    Science. 1977 Dec 9;198(4321):1041-6 PMID: 929185
  13. A genetic analysis of the att-int-xis region of coliphage lambda.
    J Mol Biol. 1977 Apr;111(2):97-120 PMID: 859185
  14. The use of specialised transducing phages in the amplification of enzyme production.
    Mol Gen Genet. 1976 Nov 24;149(1):87-99 PMID: 796672
  15. Characterization of lambdatrp-transducing bacteriophages made in vitro.
    J Mol Biol. 1976 Nov 15;107(4):549-69 PMID: 794488
  16. Improved derivative of a phage lambda EK2 vector for cloning recombinant DNA.
    Nature. 1976 Oct 7;263(5577):526-7 PMID: 1067476
  17. Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi.
    J Mol Biol. 1971 Mar 14;56(2):403-23 PMID: 5551396
  18. Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3370-4 PMID: 4343968
  19. Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579-83 PMID: 4216019
  20. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.
    Nature. 1974 Oct 11;251(5475):476-81 PMID: 4608939
  21. Specialized transducing phages carrying fusions of the trp and lac regions of the E. coli chromosome.
    Mol Gen Genet. 1974;131(1):9-19 PMID: 4605133
  22. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  23. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.
    Nature. 1973 Jan 12;241(5385):120-3 PMID: 4572006
  24. The construction of lambda transducing phages containing deletions defining regulatory elements of the lac and trp operons in E. coli.
    Mol Gen Genet. 1974 Mar 27;129(3):201-15 PMID: 4601264
  25. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2534-8 PMID: 4601822
  26. Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):3927-30 PMID: 4530273
  27. Physical mapping of the att-N region of coliphage lambda: apparent oversaturation of coding capacity in the gam-ral segment.
    Biochimie. 1974;56(11-12):1497-503 PMID: 4466498
  28. Deletions and insertions in the immunity region of coliphage lambda: revised measurement of the promoter-startpoint distance.
    Virology. 1974 Dec;62(2):458-71 PMID: 4432374
  29. Hybrids of bacteriophages lambda and phi 80: a study of nonvegetative functions.
    Virology. 1969 Apr;37(4):585-96 PMID: 4976535
  30. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages.
    J Bacteriol. 1971 Oct;108(1):5-9 PMID: 4941573
  31. Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):2977-81 PMID: 4943550
  32. Nonessential functions of bacteriophage lambda.
    Virology. 1969 Feb;37(2):177-88 PMID: 4974326
  33. [Mutational loss of the B-specific restriction in bacteriophage fd].
    Pathol Microbiol (Basel). 1967;30(6):946-52 PMID: 4873409
  34. Nonsense codons and polarity in the tryptophan operon.
    J Mol Biol. 1966 Nov 14;21(2):313-34 PMID: 5339605
  35. Mutants of Escherichia coli unable to be lysogenized by the temperate bacteriophage P2.
    Virology. 1969 Feb;37(2):163-76 PMID: 4884707
  36. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
    Virology. 1969 May;38(1):200-2 PMID: 4891223
  37. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.
    J Mol Biol. 1966 Mar;16(1):118-33 PMID: 5331240
  38. The construction in vitro of transducing derivatives of phage lambda.
    Mol Gen Genet. 1976 Jul 23;146(2):199-207 PMID: 785220
  39. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  40. The nature and origin of a class of essential gene substitutions in bacteriophage lambda.
    Virology. 1975 Sep;67(1):124-35 PMID: 1099781
  41. Hybridization of labeled RNA to DNA in agarose gels.
    Nucleic Acids Res. 1975 Oct;2(10):1911-29 PMID: 1103090
  42. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
    J Mol Biol. 1975 Nov 5;98(3):551-64 PMID: 1104875
  43. Safer derivatives of bacteriophage lambdagt-lambdaC for use in cloning of recombinant DNA molecules.
    Nature. 1976 Feb 19;259(5544):596-8 PMID: 765843
  44. The red plaque test: a rapid method for identification of excision defective variants of bacteriophage lambda.
    Virology. 1976 Jul 1;72(1):147-53 PMID: 779239
  45. A deletion analysis of prophage lambda and adjacent genetic regions.
    Proc Natl Acad Sci U S A. 1968 Nov;61(3):956-62 PMID: 5246560
  46. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination.
    J Mol Biol. 1968 Jul 14;34(2):261-71 PMID: 5760458
  47. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.
    Science. 1969 Mar 21;163(3873):1343-8 PMID: 5765116
  48. Studies on the genetics of biotin-transducing, defective variants of bacteriophage lambda.
    Virology. 1968 Sep;36(1):30-41 PMID: 5669988
  49. Integration-negative mutants of bacteriophage lambda.
    J Mol Biol. 1968 Feb 14;31(3):487-505 PMID: 5637199
  50. The c-region of coliphage 21.
    Virology. 1967 Jul;32(3):475-81 PMID: 6028942
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.2.555-575.1979
Published
1979-02-00
Pages
555-75
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353190
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.2.555-575.1979 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2