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PMID: 3628004 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A growth-related mRNA in cultured mouse cells encodes a placental calcium binding protein.

Nucleic acids research ·Vol. 15 ·No. 16 ·1987-08-25 ·Pages 6677-90

Jackson-Grusby LL, Swiergiel J, Linzer DI

Abstract

We have characterized an mRNA that increases in abundance after serum stimulation of quiescent mouse fibroblasts. This mRNA, designated 18A2, encodes a predicted polypeptide of 101 amino acids with homology to known calcium binding proteins. A variety of mouse tissues express the 18A2 mRNA, with the highest levels detected in the non-pregnant uterus and in the placenta. The concentration of 18A2 mRNA in total placental RNA decreases from day 8 to day 10 of pregnancy, and is below detection throughout the latter half of gestation. In serum-stimulated fibroblasts, the increase in 18A2 mRNA is dependent on protein synthesis. The 18A2 mRNA is similar in size, serum-inducibility, and sequence to the 2A9 mRNA (1), but these mRNAs are derived from distinct genes. This suggests that the mouse genome harbors a family of serum-inducible genes encoding proteins predicted to bind calcium.

MeSH Terms
Animals Blood Physiological Phenomena Calcium-Binding Proteins/genetics Cattle Cell Division/drug effects Cell Line Culture Media/pharmacology Female Fibroblasts/analysis Mice Mice, Inbred C3H Placenta/analysis Pregnancy Pregnancy Proteins/genetics RNA, Messenger/genetics Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Calcium-Binding Proteins Culture Media Pregnancy Proteins RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jackson-Grusby L L
Swiergiel J
Linzer D I
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41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.16.6677
Published
1987-08-25
Pages
6677-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306131
Subset
IM
Grants
NIGMS NIH HHS · GM 34238 · United States
Databases
GENBANK
X05835
External Links
PubMed source DOI: 10.1093/nar/15.16.6677 PMC Full Text View journal details
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