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PMID: 3741407 Published · ppublish English Journal Article

The purification and complete amino acid sequence of the 9000-Mr Ca2+-binding protein from rat placenta. Identity with the vitamin D-dependent intestinal Ca2+-binding protein.

The Biochemical journal ·Vol. 235 ·No. 2 ·1986-04-15 ·Pages 585-95

MacManus JP, Watson DC, Yaguchi M

Abstract

A 9000-Mr Ca2+-binding protein was isolated from rat placenta and purified to homogeneity by h.p.l.c. procedures. The complete amino acid sequence was established for the 78-residue placental protein. A sequence analysis of a minor component of the rat intestinal Ca2+-binding protein (residues 4-78) and a tryptic peptide (residues 55-74), both purified by h.p.l.c., showed both proteins to be identical. Thus this placental 9000-Mr Ca2+-binding protein is the same gene product as the intestinal Ca2+-binding protein whose synthesis is dependent on vitamin D.

MeSH Terms
Amino Acid Sequence Animals Calcium-Binding Proteins/isolation & purification Chromatography, High Pressure Liquid Female Intestinal Mucosa/analysis Peptide Fragments/analysis Placenta/analysis Pregnancy Rats Rats, Inbred Strains S100 Calcium Binding Protein G/isolation & purification
Chemicals
Calcium-Binding Proteins Peptide Fragments S100 Calcium Binding Protein G
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
MacManus J P
Watson D C
Yaguchi M
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45 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1986-04-15
Pages
585-95
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1146724
Subset
IM
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