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PMID: 3841805 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of a cDNA clone encoding murine mitogen-regulated protein: regulation of mRNA levels in mortal and immortal cell lines.

Molecular and cellular biology ·Vol. 5 ·No. 11 ·1985-11-00 ·Pages 3289-92

Parfett CL, Hamilton RT, Howell BW, Edwards DR, Nilsen-Hamilton M, Denhardt DT

Abstract

Mitogen-regulated protein (MRP) is secreted by certain immortal murine cell lines (Swiss 3T3, BNL) stimulated with serum or particular growth factors. We have identified a cDNA clone that encodes part of the protein and have confirmed that MRP is closely related to, if not identical to, the prolactin-related protein designated proliferin. MRP is not produced by primary mouse embryo fibroblasts to nearly the same extent as it is produced by many immortal or transformed lines. Control of expression of this protein by growth factors is achieved both by regulating the extent of transcription and by regulating the processing of the protein.

MeSH Terms
Animals Cell Line Cells, Cultured Cloning, Molecular DNA/isolation & purification Glycoproteins/genetics Homeostasis Intercellular Signaling Peptides and Proteins Mice Nucleic Acid Hybridization Prolactin RNA, Messenger/genetics,metabolism Transcription, Genetic
Chemicals
Glycoproteins Intercellular Signaling Peptides and Proteins Prl2c2 protein, mouse RNA, Messenger Prolactin DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Parfett C L
Hamilton R T
Howell B W
Edwards D R
Nilsen-Hamilton M
Denhardt D T
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15 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.11.3289-3292.1985
Published
1985-11-00
Pages
3289-92
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369146
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.5.11.3289-3292.1985 PMC Full Text View journal details
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