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PMID: 3624309 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Biosynthesis and interconversion of Drosophila nuclear lamin isoforms during normal growth and in response to heat shock.

The Journal of cell biology ·Vol. 105 ·No. 2 ·1987-08-00 ·Pages 771-90

Smith DE, Gruenbaum Y, Berrios M, Fisher PA

Abstract

Two major immunocross-reactive polypeptides of the Drosophila nuclear envelope, distinguishable in interphase cells on the basis of one-dimensional SDS-PAGE mobility, have been localized to the nuclear lamina by immunoelectron microscopy. These have been designated lamins Dm1 and Dm2. Both lamins are apparently derived posttranslationally from a single, primary translation product, lamin Dm0. A pathway has been established whereby lamin Dm0 is processed almost immediately upon synthesis in the cytoplasm to lamin Dm1. Processing occurs posttranslationally, is apparently proteolytic, and has been reconstituted from cell-free extracts in vitro. Processing in vitro is ATP dependent. Once assembled into the nuclear envelope, a portion of lamin Dm1 is converted into lamin Dm2 by differential phosphorylation. Throughout most stages of development and in Schneider 2 tissue culture cells, both lamin isoforms are present in approximately equal abundance. However, during heat shock, lamin Dm2 is converted nearly quantitatively into lamin Dm1. Implications for understanding the regulation of nuclear lamina plasticity through normal growth and in response to heat shock are discussed.

MeSH Terms
Animals Cloning, Molecular Drosophila/embryology,physiology Drosophila Proteins Embryo, Nonmammalian Heat-Shock Proteins/genetics Hot Temperature Lamins Nuclear Envelope/metabolism,ultrastructure Nucleoproteins/biosynthesis,genetics,metabolism Protein Biosynthesis Transcription, Genetic
Chemicals
Drosophila Proteins Heat-Shock Proteins Lam protein, Drosophila Lamins Nucleoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith D E
Gruenbaum Y
Berrios M
Fisher P A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.2.771
Published
1987-08-00
Pages
771-90
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114747
Subset
IM
Grants
NIGMS NIH HHS · GM-07518 · United States
NIGMS NIH HHS · GM-33132 · United States
External Links
PubMed source DOI: 10.1083/jcb.105.2.771 PMC Full Text View journal details
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