Home LiteratureArticle Details
PMID: 3462705 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 17 ·1986-09-00 ·Pages 6450-4

Fisher DZ, Chaudhary N, Blobel G

Abstract

The amino acid sequences deduced from cDNA clones of human lamin A and lamin C show identity between these two lamins except for an extra 9.0-kDa carboxyl-terminal tail that is present only in lamin A. Both lamins A and C contain an alpha-helical domain of approximately 360 residues that shows striking homology to a corresponding alpha-helical rod domain that is the structural hallmark of all intermediate filament proteins. However, the lamin alpha-helical domain is 14% larger than that of the intermediate filament proteins. In addition to the extensive homology to intermediate filament proteins as reported [McKeon, F., Kirschner, M. & Caput, D. (1986) Nature (London) 319, 463-468], a different 82-amino acid residue stretch at the carboxyl terminus of lamin A has been deduced and verified by amino acid sequencing. This region contains sequence homology to amino- and carboxylterminal domains of type I and type II epidermal keratins. Implications of the presence of these and other domains in lamins A and C for the assembly of the nuclear lamina are discussed.

MeSH Terms
Amino Acid Sequence Animals Cell Nucleus/ultrastructure Cloning, Molecular DNA/genetics Humans Intermediate Filament Proteins/genetics Lamin Type A Lamins Nucleoproteins/genetics,isolation & purification Rats Sequence Homology, Nucleic Acid
Chemicals
Intermediate Filament Proteins Lamin Type A Lamins Nucleoproteins lamin C DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fisher D Z
Chaudhary N
Blobel G
References (36)
36 references, click to expand
  1. Localization of a nuclear envelope-associated protein by indirect immunofluorescence microscopy using antibodies against a major polypeptide from rat liver fractions enriched in nuclear envelope-associated material.
    Cytobiologie. 1978 Oct;18(1):22-38 PMID: 361462
  2. Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis.
    J Biol Chem. 1986 Mar 25;261(9):4229-38 PMID: 3949810
  3. Prediction of the secondary structure of proteins from their amino acid sequence.
    Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 PMID: 364941
  4. The nuclear envelope lamina is reversibly depolymerized during mitosis.
    Cell. 1980 Jan;19(1):277-87 PMID: 7357605
  5. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  6. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  7. Differentiation-related patterns of expression of proteins of intermediate-size filaments in tissues and cultured cells.
    Cold Spring Harb Symp Quant Biol. 1982;46 Pt 1:431-53 PMID: 7049531
  8. The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins.
    Cell. 1982 Nov;31(1):243-52 PMID: 6186381
  9. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
    Mol Cell Biol. 1983 Feb;3(2):280-9 PMID: 6300662
  10. Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments.
    Nature. 1983 Apr 28;302(5911):794-800 PMID: 6188955
  11. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  12. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  13. Unraveling the structure of the intermediate filaments.
    Cell. 1983 Sep;34(2):332-4 PMID: 6352049
  14. RNA splicing: three themes with variations.
    Cell. 1983 Oct;34(3):713-6 PMID: 6354470
  15. The structure of the vimentin gene.
    Cell. 1983 Nov;35(1):215-23 PMID: 6194898
  16. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  17. Three different fibronectin mRNAs arise by alternative splicing within the coding region.
    Cell. 1983 Dec;35(2 Pt 1):421-31 PMID: 6317187
  18. A monoclonal antibody against nuclear lamina proteins reveals cell type-specificity in Xenopus laevis.
    Exp Cell Res. 1984 Jan;150(1):47-59 PMID: 6198191
  19. Identification of distinct messenger RNAs for nuclear lamin C and a putative precursor of nuclear lamin A.
    J Cell Biol. 1984 Mar;98(3):980-5 PMID: 6699095
  20. The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 Da: analysis of sequence differences between type I and type II keratins.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5709-13 PMID: 6207530
  21. A short amino acid sequence able to specify nuclear location.
    Cell. 1984 Dec;39(3 Pt 2):499-509 PMID: 6096007
  22. Hybrid character of a large neurofilament protein (NF-M): intermediate filament type sequence followed by a long and acidic carboxy-terminal extension.
    EMBO J. 1984 Nov;3(11):2701-6 PMID: 6439558
  23. Organization and modulation of nuclear lamina structure.
    J Cell Sci Suppl. 1984;1:137-60 PMID: 6597817
  24. Structure of a gene for the human epidermal 67-kDa keratin.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1896-900 PMID: 2580302
  25. A monoclonal antibody which recognises each of the nuclear lamin polypeptides in mammalian cells.
    EMBO J. 1983;2(3):361-7 PMID: 11894950
  26. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  27. Isolation of nuclear pore complexes in association with a lamina.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1007-11 PMID: 1055359
  28. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  29. A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
    J Cell Biol. 1976 Sep;70(3):581-91 PMID: 986398
  30. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  31. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  32. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  33. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
    Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
  34. The nuclear lamins. A multigene family of proteins in evolution and differentiation.
    Exp Cell Res. 1986 Jan;162(1):1-10 PMID: 2415378
  35. Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
    Nature. 1986 Feb 6-12;319(6053):463-8 PMID: 3453101
  36. Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
    J Cell Biol. 1978 Nov;79(2 Pt 1):546-66 PMID: 102651
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.17.6450
Published
1986-09-00
Pages
6450-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386521
Subset
IM
Grants
NIGMS NIH HHS · GM07982 · United States
NIGMS NIH HHS · GM27155 · United States
Databases
GENBANK
M13451, M13452
External Links
PubMed source DOI: 10.1073/pnas.83.17.6450 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2