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PMID: 6192431 Published · ppublish English Case Reports Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Autoimmune response directed against conserved determinants of nuclear envelope proteins in a patient with linear scleroderma.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 14 ·1983-07-00 ·Pages 4374-8

McKeon FD, Tuffanelli DL, Fukuyama K, Kirschner MW

Abstract

We have studied the autoantibodies in the serum of a patient with linear scleroderma that specifically recognize the nuclear envelope of cultured cells. These antibodies bind to conserved determinants of nuclear lamins, the predominant mammalian nuclear envelope proteins. Of the three mammalian nuclear lamin proteins (970, P68, and P60), only P70 and P60 bind the autoantibodies. In addition, two proteins of the Drosophila embryonic nuclear matrix, P70 and P68, bind these autoantibodies. We have used nuclear matrices to isolate the autoantibodies from the patient's serum that react to the nuclear lamins. At least three different IgG heavy chains were found to be involved in this autoimmune response to nuclear lamins, indicating that this response is not due to the expansion of a single B-cell clone.

MeSH Terms
Adult Antigens, Surface/analysis Autoantibodies/analysis Autoimmune Diseases/immunology Epitopes/analysis Female Fluorescent Antibody Technique Humans Immunoenzyme Techniques Membrane Proteins/immunology Nuclear Envelope/immunology Scleroderma, Localized/immunology Skin/immunology
Chemicals
Antigens, Surface Autoantibodies Epitopes Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McKeon F D
Tuffanelli D L
Fukuyama K
Kirschner M W
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.14.4374
Published
1983-07-00
Pages
4374-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384040
Subset
IM
Grants
NIADDK NIH HHS · AM12433 · United States
NIGMS NIH HHS · GM26875 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.14.4374 PMC Full Text View journal details
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