Home LiteratureArticle Details
PMID: 6966403 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Autoantibody to centromere (kinetochore) in scleroderma sera.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 3 ·1980-03-00 ·Pages 1627-31

Moroi Y, Peebles C, Fritzler MJ, Steigerwald J, Tan EM

Abstract

Sera from patients with scleroderma contained several autoantibodies to nuclear antigens which were distinguished by different patterns of nuclear immunofluorescence staining. One of these autoantibodies reacted with centromeric regions of chromosomes. In chromosome spreads, the staining appeared as two small spheres at the centromere, resembling kinetochores. The antigenic determinant appeared to be a protein or polypeptide tightly bound to DNA. The autoantibody was reactive with centromeres of cells derived from humans, mice, and Chinese hamsters. The autoantibody was present in high frequency in the calcinosis/Raynaud's phenomenon/esophageal dysmotility/sclerodactyly/telangiectasia variant (CREST) of scleroderma.

MeSH Terms
Antibodies, Antinuclear/analysis Autoantibodies/analysis Centromere/immunology Chromosomes/immunology Female Humans Male Nucleoproteins/immunology Scleroderma, Systemic/immunology
Chemicals
Antibodies, Antinuclear Autoantibodies Nucleoproteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moroi Y
Peebles C
Fritzler M J
Steigerwald J
Tan E M
References (19)
19 references, click to expand
  1. Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus.
    J Immunol. 1966 Mar;96(3):464-71 PMID: 5932578
  2. The immunofluorescent tumor imprint technique. II. The frequency of antinuclear factors in connective tissue diseases and dermatoses.
    Ann Intern Med. 1966 Jul;65(1):9-19 PMID: 4160797
  3. Relationship of nuclear staining patterns with precipitating antibodies in systemic lupus erythematosus.
    J Lab Clin Med. 1967 Nov;70(5):800-12 PMID: 4964289
  4. Serum antinuclear antibodies in progressive systemic sclerosis (scleroderma).
    Arthritis Rheum. 1968 Oct;11(5):607-17 PMID: 4176069
  5. An immunological study of the fates of nuclear and nucleolar macromoleculus during the cell cycle.
    J Mol Biol. 1972 Jul 14;68(1):107-14 PMID: 4115106
  6. Antinucleolar antibodies in human sera.
    J Immunol. 1973 Oct;111(4):996-1004 PMID: 4199560
  7. New selective Giemsa technique for human chromosomes, Cd staining.
    Nature. 1974 Mar 1;248(5443):55 PMID: 4131938
  8. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
    Int J Cancer. 1973 May;11(3):499-520 PMID: 4133943
  9. Combined titre and fluorescent pattern of IgG antinuclear antibodies using cultured cell monolayers in evaluating connective tissue diseases.
    Ann Rheum Dis. 1974 Sep;33(5):465-72 PMID: 4608364
  10. Dissection of chromosome structure with trypsin and nucleases.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4249-53 PMID: 4530301
  11. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy.
    J Histochem Cytochem. 1974 Dec;22(12):1077-83 PMID: 4374474
  12. Release of discrete subunits after nuclease and trypsin digestion of chromatin.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1212-6 PMID: 1055376
  13. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection.
    Intervirology. 1975;5(6):319-34 PMID: 181343
  14. Studies on antibodies to histones by immunofluorescence.
    Scand J Immunol. 1976;5(6-7):811-8 PMID: 62391
  15. Non-histone protein associated with centromeric heterochromatin in the mouse chromosome.
    Exp Cell Res. 1977 Mar 1;105(1):217-22 PMID: 837995
  16. Nuclear proteins. IV. Deficiency of non-histone proteins in condensed chromatin of Drosophila virilis and mouse.
    Exp Cell Res. 1977 Mar 15;105(2):349-65 PMID: 66151
  17. Recent progress in the study of autoantibodies to nuclear antigens.
    Hum Pathol. 1978 Jan;9(1):85-91 PMID: 344192
  18. Identification of a nuclear protein (Scl-70) as a unique target of human antinuclear antibodies in scleroderma.
    J Biol Chem. 1979 Oct 25;254(20):10514-22 PMID: 385602
  19. Antinucleolar antibodies.
    Lancet. 1962 Sep 22;2(7256):575-7 PMID: 13866283
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.3.1627
Published
1980-03-00
Pages
1627-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348550
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.3.1627 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2