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PMID: 3541892 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Site-directed mutagenesis.

The Biochemical journal ·Vol. 237 ·No. 1 ·1986-07-01 ·Pages 1-7

Carter P

Abstract

暂无摘要

MeSH Terms
Binding Sites DNA, Single-Stranded/genetics Deoxyuridine/genetics Genetic Vectors Mutation Nucleic Acid Hybridization Oligonucleotides/chemical synthesis,genetics Templates, Genetic
Chemicals
DNA, Single-Stranded Oligonucleotides Deoxyuridine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Carter P
References (76)
76 references, click to expand
  1. In vitro mutagenesis.
    Annu Rev Genet. 1985;19:423-62 PMID: 3002241
  2. Altered Cro repressors from engineered mutagenesis of a synthetic cro gene.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1084-8 PMID: 3156377
  3. Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing.
    Gene. 1984 Feb;27(2):183-91 PMID: 6327466
  4. The use of bacteriophage M13 carrying defined fragments of the Escherichia coli gltA gene to determine the location and structure of the citrate synthase promoter region.
    Mol Gen Genet. 1983;191(3):499-506 PMID: 6355771
  5. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.
    Methods Enzymol. 1983;100:468-500 PMID: 6225933
  6. Sequencing of large double-stranded DNA using the dideoxy sequencing technique.
    Biosci Rep. 1982 Nov;2(11):907-12 PMID: 6218841
  7. EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesis.
    Nucleic Acids Res. 1985 Dec 9;13(23):8561-71 PMID: 3001643
  8. Convenient modification of the method for oligonucleotide-directed in vitro mutagenesis of cloned DNA.
    FEBS Lett. 1985 Feb 25;181(2):407-11 PMID: 3882458
  9. Site-specific mutagenesis of the human interleukin-2 gene: structure-function analysis of the cysteine residues.
    Science. 1984 Jun 29;224(4656):1431-3 PMID: 6427925
  10. Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum ologodeoxyribonucleotide length.
    Gene. 1979 Dec;8(1):81-97 PMID: 161245
  11. Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.
    J Virol. 1984 Dec;52(3):892-6 PMID: 6238174
  12. Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5' of an ATG initiation codon.
    Nucleic Acids Res. 1983 May 25;11(10):3113-21 PMID: 6304623
  13. Sequence specificity of the P1 modification methylase (M.Eco P1) and the DNA methylase (M.Eco dam) controlled by the Escherichia coli dam gene.
    J Mol Biol. 1978 Dec 15;126(3):367-80 PMID: 370402
  14. Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6409-13 PMID: 6983070
  15. Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.
    EMBO J. 1984 Mar;3(3):623-9 PMID: 6425057
  16. Primer-directed mutagenesis of linearized plasmids.
    Anal Biochem. 1984 Dec;143(2):341-9 PMID: 6397999
  17. Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):851-5 PMID: 191836
  18. Construction of viable and lethal mutations in the origin of bacteriophage 'phi' X174 using synthetic oligodeoxyribonucleotides.
    J Mol Biol. 1981 Nov 15;152(4):615-39 PMID: 6460870
  19. Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli.
    Cell. 1984 Oct;38(3):879-87 PMID: 6386179
  20. The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.
    Nucleic Acids Res. 1985 Dec 20;13(24):8749-64 PMID: 3001649
  21. Minimal size plasmids containing an M13 origin for production of single-strand transducing particles.
    J Mol Appl Genet. 1984;2(6):507-17 PMID: 6099398
  22. Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4753-7 PMID: 6170979
  23. Chemical synthesis and expression of a calmodulin gene designed for site-specific mutagenesis.
    Biochemistry. 1985 Sep 10;24(19):5090-8 PMID: 3000422
  24. Substituting an alpha-helix switches the sequence-specific DNA interactions of a repressor.
    Cell. 1984 Sep;38(2):361-9 PMID: 6540625
  25. Filter disc supported oligonucleotide synthesis by the phosphite method.
    Nucleic Acids Res. 1984 Dec 11;12(23):9137-42 PMID: 6514575
  26. Effect of deoxynucleoside phosphorothioates incorporated in DNA on cleavage by restriction enzymes.
    J Biol Chem. 1982 Jun 10;257(11):6595-9 PMID: 6281279
  27. Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale.
    EMBO J. 1984 Apr;3(4):801-5 PMID: 16453516
  28. The gapped duplex DNA approach to oligonucleotide-directed mutation construction.
    Nucleic Acids Res. 1984 Dec 21;12(24):9441-56 PMID: 6096830
  29. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  30. Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding.
    Nature. 1982 Oct 21;299(5885):756-8 PMID: 6811955
  31. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  32. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.
    Nucleic Acids Res. 1985 Dec 20;13(24):8765-85 PMID: 3001650
  33. Active site of triosephosphate isomerase: in vitro mutagenesis and characterization of an altered enzyme.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2272-6 PMID: 3887397
  34. Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.
    DNA. 1984 Dec;3(6):479-88 PMID: 6096101
  35. Total synthesis and cloning of a gene coding for the ribonuclease S protein.
    Science. 1984 Mar 23;223(4642):1299-301 PMID: 6322300
  36. Oligonucleotide directed mutagenesis: selection of mutants by hemimethylation of GATC-sequences.
    Mol Gen Genet. 1984;195(1-2):126-33 PMID: 6092841
  37. Gene synthesis machines: DNA chemistry and its uses.
    Science. 1985 Oct 18;230(4723):281-5 PMID: 3863253
  38. Mutagenesis at a specific position in a DNA sequence.
    J Biol Chem. 1978 Sep 25;253(18):6551-60 PMID: 681366
  39. A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports.
    Nucleic Acids Res. 1983 Jul 11;11(13):4365-77 PMID: 6306587
  40. Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments.
    Nucleic Acids Res. 1982 Oct 25;10(20):6475-85 PMID: 6294606
  41. Strategies and applications of in vitro mutagenesis.
    Science. 1985 Sep 20;229(4719):1193-201 PMID: 2994214
  42. Cleavage of phosphorothioate-substituted DNA by restriction endonucleases.
    J Biol Chem. 1984 Nov 25;259(22):14243-8 PMID: 6094546
  43. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.
    Nucleic Acids Res. 1982 Oct 25;10(20):6487-500 PMID: 6757864
  44. Is sequence conservation in interferons due to selection for functional proteins?
    Nature. 1985 Feb 21-27;313(6004):698-700 PMID: 3856100
  45. Redesigning trypsin: alteration of substrate specificity.
    Science. 1985 Apr 19;228(4697):291-7 PMID: 3838593
  46. In vitro generation of specific deletions in DNA cloned in M13 vectors using synthetic oligodeoxyribonucleotides: mutants in the 5'-flanking region of the yeast alcohol dehydrogenase II gene.
    Nucleic Acids Res. 1984 Mar 12;12(5):2407-19 PMID: 6324118
  47. Synthesis and use of synthetic oligonucleotides.
    Annu Rev Biochem. 1984;53:323-56 PMID: 6383196
  48. Directed deletion of a yeast transfer RNA intervening sequence.
    Science. 1980 Sep 19;209(4463):1396-400 PMID: 6997991
  49. Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13.
    Nucleic Acids Res. 1982 Feb 11;10(3):821-32 PMID: 6278437
  50. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12.
    Mutat Res. 1975 Apr;28(1):15-26 PMID: 167279
  51. Genetic studies with heteroduplex DNA of bacteriophage fl. Asymmetric segregation, base correction and implications for the mechanism of genetic recombination.
    J Mol Biol. 1975 Aug 15;96(3):495-509 PMID: 1100850
  52. A genetic enrichment for mutations constructed by oligodeoxynucleotide-directed mutagenesis.
    Gene. 1985;37(1-3):73-81 PMID: 4054630
  53. pEMBL: a new family of single stranded plasmids.
    Nucleic Acids Res. 1983 Mar 25;11(6):1645-55 PMID: 6300771
  54. A site-specific mutation within the active site of ribulose-1,5-bisphosphate carboxylase of Rhodospirillum rubrum.
    EMBO J. 1984 Dec 1;3(12):2737-43 PMID: 16453576
  55. Efficient site-directed mutagenesis by simultaneous use of two primers.
    Nucleic Acids Res. 1983 Aug 11;11(15):5103-12 PMID: 6308572
  56. Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2285-9 PMID: 16593452
  57. Oligonucleotide-directed mutagenesis by microscale 'shot-gun' gene synthesis.
    Nucleic Acids Res. 1985 May 10;13(9):3305-16 PMID: 3889852
  58. Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites.
    Gene. 1985;34(2-3):315-23 PMID: 3891521
  59. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6 PMID: 333444
  60. Conversion of closed circular DNA molecules to single-nicked molecules by digestion with DNAase I in the presence of ethidium bromide.
    Biochim Biophys Acta. 1975 Oct 15;407(3):365-75 PMID: 1186174
  61. Transforming activity of polyoma virus middle-T antigen probed by site-directed mutagenesis.
    Nature. 1983 Aug 4-10;304(5925):456-9 PMID: 6308461
  62. A frameshift mutation affecting the carboxyl terminus of the simian virus 40 large tumor antigen results in a replication- and transformation-defective virus.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7065-9 PMID: 6316342
  63. In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.
    Nucleic Acids Res. 1983 Dec 20;11(24):8595-608 PMID: 6324090
  64. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
    Cell. 1979 Apr;16(4):753-61 PMID: 222467
  65. Use of unpurified synthetic deoxynucleotide primers for rapid dideoxynucleotide chain termination sequencing.
    DNA. 1984 Aug;3(4):339-43 PMID: 6489095
  66. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  67. Directed semisynthetic point mutational analysis of an RNA polymerase III promoter.
    Nucleic Acids Res. 1983 Nov 25;11(22):7695-700 PMID: 6647034
  68. Gene K of bacteriophage phi X174 codes for a protein which affects the burst size of phage production.
    J Virol. 1985 Feb;53(2):708-9 PMID: 3155803
  69. The role of the beta-lactamase signal sequence in the secretion of proteins by Escherichia coli.
    J Biol Chem. 1984 Feb 25;259(4):2149-54 PMID: 6365904
  70. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  71. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  72. A large increase in enzyme-substrate affinity by protein engineering.
    Nature. 1984 Jan 12-18;307(5947):187-8 PMID: 6690998
  73. Improved oligonucleotide site-directed mutagenesis using M13 vectors.
    Nucleic Acids Res. 1985 Jun 25;13(12):4431-43 PMID: 2989795
  74. Targeted point mutation that creates a unique Eco RI site within the signal codons of the beta-lactamase gene without altering enzyme secretion or processing.
    J Biol Chem. 1982 Jul 25;257(14):7930-2 PMID: 6282862
  75. Directed mutagenesis of dihydrofolate reductase.
    Science. 1983 Nov 18;222(4625):782-8 PMID: 6356360
  76. Oligonucleotide-directed mutagenesis using plasmid DNA templates and two primers.
    DNA. 1984 Dec;3(6):469-77 PMID: 6096100
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2370001
Published
1986-07-01
Pages
1-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1146940
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2370001 PMC Full Text View journal details
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