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PMID: 6322300 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Total synthesis and cloning of a gene coding for the ribonuclease S protein.

Science (New York, N.Y.) ·Vol. 223 ·No. 4642 ·1984-03-23 ·Pages 1299-301

Nambiar KP, Stackhouse J, Stauffer DM, Kennedy WP, Eldredge JK, Benner SA

Abstract

A gene for ribonuclease S protein, has been chemically synthesized and cloned. The gene is designed to have 25 specific restriction endonuclease sites spaced at short intervals, permitting its structure to be rapidly modified. This flexibility facilitates tests of hypotheses relating the primary structure of the enzyme to its physical and catalytic behavior.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes, Synthetic Oligodeoxyribonucleotides/chemical synthesis Peptide Fragments/genetics Ribonucleases/genetics

Chemicals

Oligodeoxyribonucleotides Peptide Fragments ribonuclease S-peptide Ribonucleases DNA Restriction Enzymes

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Nambiar K P

Stackhouse J

Stauffer D M

Kennedy W P

Eldredge J K

Benner S A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.6322300

Published

1984-03-23

Pages

1299-301

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NIGMS NIH HHS · 1 RO1 GM 30110-01A2 · United States

Databases

GENBANK

K02873

External Links

PubMed source DOI: 10.1126/science.6322300 View journal details

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Total synthesis and cloning of a gene coding for the ribonuclease S protein.

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