Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.
Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without affecting the amino acid, glutamine, encoded by the overlapping gene D. The phi X174rb(E)557 mutant does not lyse infected Escherichia coli C and therefore results in the accumulation of a large number intracellular mature phage particles. Thus, the mutation inactivates production of the gene E lytic product, presumably by blocking translation of gene E, without affecting other phage functions.
18 references, click to expand
-
The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene.J Mol Biol. 1966 Jul;18(3):429-47 PMID: 5968177
-
Cold-sensitive mutants of bacteriophage phi-X-174. I. A mutant blocked in the eclipse function at low temperature.Proc Natl Acad Sci U S A. 1967 Sep;58(3):958-61 PMID: 5233852
-
Mutant of phi X174 accessible to host-controlled modification.J Virol. 1969 May;3(5):541-2 PMID: 4891753
-
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
-
How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
-
Overlapping genes in bacteriophage phiX174.Nature. 1976 Nov 4;264(5581):34-41 PMID: 1004533
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
The use of thin acrylamide gels for DNA sequencing.FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
-
Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence.J Biol Chem. 1978 Apr 25;253(8):2532-9 PMID: 632285
-
Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein.Proc Natl Acad Sci U S A. 1978 Jun;75(6):2741-5 PMID: 275843
-
Mutagenesis at a specific position in a DNA sequence.J Biol Chem. 1978 Sep 25;253(18):6551-60 PMID: 681366
-
Identification of lysis protein E of bacteriophage phiX174.J Virol. 1978 Oct;28(1):408-10 PMID: 702655
-
The nucleotide sequence of bacteriophage phiX174.J Mol Biol. 1978 Oct 25;125(2):225-46 PMID: 731693
-
Defined transversion mutations at a specific position in DNA using synthetic oligodeoxyribonucleotides as mutagens.Nucleic Acids Res. 1979 Jul 11;6(9):2973-85 PMID: 158749
-
Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum ologodeoxyribonucleotide length.Gene. 1979 Dec;8(1):81-97 PMID: 161245
-
Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent phi X174 mutant, with a specific nucleotide deletion, at very high efficiency.Gene. 1980 Dec;12(1-2):129-37 PMID: 6452306
-
Translational initiation in prokaryotes.Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
-
Analysis in vivo of translational mutants of the rIIB cistron of bacteriophage T4.J Mol Biol. 1981 Jul 5;149(3):405-32 PMID: 7031268
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
