Filter disc supported oligonucleotide synthesis by the phosphite method.

Nucleic acids research ·Vol. 12 ·No. 23 ·1984-12-11 ·Pages 9137-42

Ott J, Eckstein F

Abstract

The phosphite approach to oligodeoxynucleotide synthesis has been applied to the filter disc support. The method is exemplified here by the preparation of eight decadeoxynucleotides.

MeSH Terms

Base Sequence Chromatography, High Pressure Liquid Deoxyribonucleosides Indicators and Reagents Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Phosphates

Chemicals

Deoxyribonucleosides Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides Phosphates

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ott J

Eckstein F

References (8)

8 references, click to expand

Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.
Nucleic Acids Res. 1983 May 11;11(9):2575-84 PMID: 6856470
Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site.
Biochemistry. 1984 Jul 17;23(15):3443-53 PMID: 6087894
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.
Nucleic Acids Res. 1982 Oct 25;10(20):6243-54 PMID: 7177846
Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale.
EMBO J. 1984 Apr;3(4):801-5 PMID: 16453516
A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports.
Nucleic Acids Res. 1983 Jul 11;11(13):4365-77 PMID: 6306587
Methylphosphonates as probes of protein-nucleic acid interactions.
Nucleic Acids Res. 1984 Apr 11;12(7):3387-404 PMID: 6718253
Synthesis and use of synthetic oligonucleotides.
Annu Rev Biochem. 1984;53:323-56 PMID: 6383196
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94 PMID: 7177853

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.23.9137

Published

1984-12-11

Pages

9137-42

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320443

Subset

External Links

PubMed source DOI: 10.1093/nar/12.23.9137 PMC Full Text View journal details

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Filter disc supported oligonucleotide synthesis by the phosphite method.

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