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PMID: 6856470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.

Nucleic acids research ·Vol. 11 ·No. 9 ·1983-05-11 ·Pages 2575-84

Dörper T, Winnacker EL

Abstract

The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides.

MeSH Terms
Chemical Phenomena Chemistry Molecular Weight Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Phosphines
Chemicals
Oligodeoxyribonucleotides Oligonucleotides Phosphines
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dörper T
Winnacker E L
References (4)
4 references, click to expand
  1. Synthesis of thymidine oligonucleotides by phosphite triester intermediates.
    J Am Chem Soc. 1976 Jun 9;98(12):3655-61 PMID: 1270704
  2. Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates.
    J Am Chem Soc. 1977 May 11;99(10):3526-8 PMID: 853194
  3. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  4. Gene expression: chemical synthesis of E. coli ribosome binding sites and their use in directing the expression of mammalian proteins in bacteria.
    Nucleic Acids Res. 1982 Oct 25;10(20):6319-29 PMID: 6294598
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.9.2575
Published
1983-05-11
Pages
2575-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325909
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.9.2575 PMC Full Text View journal details
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