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PMID: 3531185 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mediation, by Saccharomyces cerevisiae translocation signals, of beta-lactamase transport through the Escherichia coli inner membrane and sensitive method for detection of signal sequences.

Journal of bacteriology ·Vol. 168 ·No. 1 ·1986-10-00 ·Pages 467-9

Roggenkamp R, Reipen G, Hollenberg CP

Abstract

Signal sequences of Saccharomyces cerevisiae invertase and alpha-factor pheromone were tested for the ability to mediate protein transport through the inner membrane of Escherichia coli by fusion to bacterial beta-lactamase lacking the signal sequence (blaS0). Both types of transformants exhibited ampicillin resistance in accordance with the transport of the fused protein to the periplasmic compartment. This compartment contained most of the beta-lactamase activity present in the cell. Therefore, the tested yeast signal sequences, which conferred translocation of their proteins across the membrane of the endoplasmic reticulum in S. cerevisiae, can provide the same function in E. coli. The screening for ampicillin resistance among blaS0 fusions provides a convenient method for the isolation of functional yeast and possibly higher eucaryotic signal sequences.

MeSH Terms
Biological Transport Cell Membrane/metabolism Cytoplasm/metabolism DNA, Recombinant Escherichia coli/metabolism Fungal Proteins/analysis,genetics,metabolism Protein Sorting Signals/analysis,genetics,metabolism Saccharomyces cerevisiae/metabolism beta-Lactamases/genetics,metabolism
Chemicals
DNA, Recombinant Fungal Proteins Protein Sorting Signals beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roggenkamp R
Reipen G
Hollenberg C P
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21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.1.467-469.1986
Published
1986-10-00
Pages
467-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213482
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.168.1.467-469.1986 PMC Full Text View journal details
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