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PMID: 3513191 Published · ppublish English Journal Article

Resistance to human respiratory syncytial virus (RSV) infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the RSV G glycoprotein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 6 ·1986-03-00 ·Pages 1906-10

Elango N, Prince GA, Murphy BR, Venkatesan S, Chanock RM, Moss B

Abstract

A cDNA copy of the G glycoprotein gene of human respiratory syncytial virus (RSV) was placed under control of a vaccinia virus promoter and inserted into the thymidine kinase locus of the vaccinia virus genome. The recombinant vaccinia virus retained infectivity and expressed a 93-kDa protein that migrated with the authentic RSV G glycoprotein upon polyacrylamide gel electrophoresis. Glycosylation of the expressed protein and transport to the cell surface were demonstrated in the absence of other RSV proteins. Cotton rats that were inoculated intradermally with the infectious recombinant virus produced serum antibody to the G glycoprotein that neutralized RSV in vitro. Furthermore, the vaccinated animals were resistant to lower respiratory tract infection upon intranasal inoculation with RSV and had reduced titers of RSV in the nose.

MeSH Terms
Animals Arvicolinae Cell Line Chlorocebus aethiops Cloning, Molecular DNA/genetics DNA, Viral/genetics Disease Susceptibility Genetic Vectors Immunization Kidney Recombinant Proteins/genetics,immunology Respiratory Syncytial Viruses/genetics,immunology Respirovirus Infections/immunology,prevention & control T-Lymphocytes, Cytotoxic/immunology Vaccinia virus/genetics Viral Proteins/genetics,immunology
Chemicals
DNA, Viral Recombinant Proteins Viral Proteins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Elango N
Prince G A
Murphy B R
Venkatesan S
Chanock R M
Moss B
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.6.1906
Published
1986-03-00
Pages
1906-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323193
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.6.1906 PMC Full Text View journal details
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