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PMID: 7107006 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion.

Infection and immunity ·Vol. 37 ·No. 1 ·1982-07-00 ·Pages 243-9

Fernie BF, Gerin JL

Abstract

We identified by immunochemical methods 13 polypeptides associated with the infectious respiratory syncytial virus virion. Eight of these polypeptides (VP200, VP84, VP66, VP43, VP40, VP37, VP28, and VP19) were identified as virus specific. Two other polypeptides, (VP) 22 and (VP) 12, are provisionally considered to be of viral origin. Three nonviral proteins are also intimately associated with the infectious virion. These nonviral proteins were identified as cellular actin and two proteins with bovine serum albumin immunospecificity. VP40 was identified as the major ribonucleoprotein. Based on biochemical and biophysical similarities with paramyxovirus proteins, other respiratory syncytial virus proteins are believed to have these specific viral functions: VP84, "hemagglutinin"; VP66, undissociated fusion protein, F1,2; VP43, F1; and VP19, F2, VP66 contains a major determinant involved in viral infectivity since all neutralizing antibodies tested, including a monoclone, precipitated this protein.

MeSH Terms
Actins/isolation & purification Animals Hemagglutinins, Viral/isolation & purification Immune Sera Precipitin Tests Respiratory Syncytial Viruses/analysis,immunology Ribonucleoproteins/isolation & purification Viral Proteins/immunology,isolation & purification Virion/analysis
Chemicals
Actins Hemagglutinins, Viral Immune Sera Ribonucleoproteins Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fernie B F
Gerin J L
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31 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.37.1.243-249.1982
Published
1982-07-00
Pages
243-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347519
Subset
IM
Grants
NIAID NIH HHS · N01-AI-82572 · United States
External Links
PubMed source DOI: 10.1128/iai.37.1.243-249.1982 PMC Full Text View journal details
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