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PMID: 3510430 Published · ppublish English Comparative Study Journal Article

Detection and identification of activated oncogenes in spontaneously occurring benign and malignant hepatocellular tumors of the B6C3F1 mouse.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 1 ·1986-01-00 ·Pages 33-7

Reynolds SH, Stowers SJ, Maronpot RR, Anderson MW, Aaronson SA

Abstract

Species- and strain-specific spontaneously occurring tumors have been observed in rodents maintained under normal laboratory conditions. Elucidation of the molecular mechanisms associated with the development of these spontaneous tumors may provide a better understanding of tumor development associated with exposure to chemical carcinogens. In view of the high frequencies of oncogene activation shown in rodent tumors induced by known chemical carcinogens, we have investigated oncogene activation in spontaneous tumors of the B6C3F1 mouse and Fischer 344/N rat by DNA transfection techniques. A marked difference in the presence of activated oncogenes in spontaneous rat tumors versus spontaneous mouse liver tumors was observed in this study. All rat tumors tested failed to yield activated oncogenes (0/29), whereas 30% (3/10) of mouse hepatocellular adenomas and 77% (10/13) of hepatocellular carcinomas scored positive by DNA transfection. These transforming genes were identified as an activated Ha-ras gene in all the adenoma transfectants and in 8 of the 10 carcinoma transfectants. The two remaining hepatocellular carcinomas contained transforming genes that appear not to be members of the known ras gene family. The B6C3F1 mouse liver system might provide a very sensitive assay not only for assessing the potential of a chemical to activate a cellular proto-oncogene, but also for detecting various classes of proto-oncogenes that are susceptible to mutational activation.

MeSH Terms
Adenoma/genetics Animals Carcinoma/genetics DNA/genetics Electrophoresis, Polyacrylamide Gel Immunologic Techniques Liver Neoplasms, Experimental/genetics Mice Nucleic Acid Hybridization Oncogenes Rats Rats, Inbred F344 Species Specificity Transfection
Chemicals
DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reynolds S H
Stowers S J
Maronpot R R
Anderson M W
Aaronson S A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.1.33
Published
1986-01-00
Pages
33-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322785
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.1.33 PMC Full Text View journal details
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