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PMID: 3510311 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Hepatitis B virus polypeptide X: expression in Escherichia coli and identification of specific antibodies in sera from hepatitis B virus-infected humans.

Journal of virology ·Vol. 57 ·No. 1 ·1986-01-00 ·Pages 101-9

Meyers ML, Trepo LV, Nath N, Sninsky JJ

Abstract

Sequence analysis of the hepatitis B virus (HBV) genome revealed the presence of an open reading frame (ORF X) which has the potential to encode a 154-amino acid polypeptide. A fusion protein containing 145 of the amino acids encoded by ORF X and 8 amino acids of beta-galactosidase was expressed and characterized in bacterial extracts. Immunoprecipitations with the ORF X fusion protein as a radioactively labeled antigen were performed to screen sera of humans infected with HBV for the presence of antibodies against ORF X-encoded determinants (anti-X). Such antibodies were identified in 9 samples from a set of 26 sera characterized as positive for HBV surface antigen but were not found in 16 normal human sera. The data reported here demonstrate that sera from some patients with markers of HBV infection contain antibodies directed against the polypeptide encoded by ORF X. As such, these findings represent evidence that ORF X constitutes a gene, or a portion of a gene, which is expressed during HBV infection. Although there does not appear to be a direct relationship between anti-X and any individual markers of HBV infection, our data suggest that anti-X is more prevalent in HBV-positive sera containing antibodies to HBe3 antigen (anti-HBe3).

MeSH Terms
Base Sequence Cell-Free System Cloning, Molecular Escherichia coli/genetics Hepatitis B Antibodies/analysis Hepatitis B virus/genetics,immunology Humans Peptides/genetics,immunology Recombinant Proteins/biosynthesis,genetics Viral Proteins/biosynthesis,genetics,immunology
Chemicals
Hepatitis B Antibodies Peptides Recombinant Proteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Meyers M L
Trepo L V
Nath N
Sninsky J J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.57.1.101-109.1986
Published
1986-01-00
Pages
101-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252703
Subset
IM
Grants
NIAID NIH HHS · AI 08295-02 · United States
NCI NIH HHS · CA09060 · United States
NCI NIH HHS · P30-CA13330 · United States
External Links
PubMed source DOI: 10.1128/JVI.57.1.101-109.1986 PMC Full Text View journal details
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