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PMID: 6089197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specific hepatitis B virus integration in hepatocellular carcinoma DNA through a viral 11-base-pair direct repeat.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 17 ·1984-09-00 ·Pages 5350-4

Dejean A, Sonigo P, Wain-Hobson S, Tiollais P

Abstract

Integrated hepatitis B virus (HBV) DNA sequences have been cloned from cellular DNA of two human liver tumors. The structure of the clones was determined by restriction mapping, and the host-viral DNA junctions were sequenced. In each clone one junction mapped to within an 11-base-pair sequence, 5' T-T-C-A-C-C-T-C-T-G-C, which is directly repeated near the extremities of the cohesive-end region of the free viral genome. The two copies of this sequence are termed DR1 and DR2. While one clone carried a host-viral junction within DR1, the second one carried a host-viral junction within DR2. The first 1 or 2 base pairs of the repeat were deleted upon recombination with the host genome, leaving at the junctions a common 9-base-pair segment of HBV DNA, 5' C-A-C-C-T-C-T-G-C. The other two host-viral junctions mapped to the pre-S region and to the core region of the viral genome, showing no peculiar feature. These results show that HBV DNA can integrate via a specific viral DNA sequence.

MeSH Terms
Base Composition Base Sequence Carcinoma, Hepatocellular/genetics,microbiology Cloning, Molecular DNA Restriction Enzymes DNA, Neoplasm/genetics DNA, Viral/genetics Hepatitis B virus/genetics Humans Liver Neoplasms/genetics,microbiology Nucleic Acid Hybridization
Chemicals
DNA, Neoplasm DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dejean A
Sonigo P
Wain-Hobson S
Tiollais P
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.17.5350
Published
1984-09-00
Pages
5350-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391701
Subset
IM
Databases
GENBANK
K02716, K02717
External Links
PubMed source DOI: 10.1073/pnas.81.17.5350 PMC Full Text View journal details
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