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PMID: 6302689 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of integrated hepatitis B viral DNA cloned from a human hepatoma and the hepatoma-derived cell line PLC/PRF/5.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 9 ·1983-05-00 ·Pages 2505-9

Dejean A, Brechot C, Tiollais P, Wain-Hobson S

Abstract

Recombinant phage clones carrying integrated hepatitis B virus (HBV) DNA sequences have been isolated from two phage libraries made from human DNA of a hepatoma and a hepatoma-derived cell line. One clone from each library has been characterized both by restriction mapping and by electron microscopy. In one clone there is at least one complete and uninterrupted HBV genome, and in the other the HBV sequences are composed of two major subgenomic fragments inverted with respect to each other. The host-virus junctions are localized within the positions 1,700-2,600 base pairs on the physical map of the free viral genome. The pre-S/S (surface antigen gene) region is conserved between the two clones. The two clones do not have common cellular sequences nor do they contain cellular homologues to six retroviral oncogenes. For one clone, the hepatitis B surface antigen gene was found to be functional when introduced into mouse thymidine kinase-negative cells by transfection.

MeSH Terms
Carcinoma, Hepatocellular/genetics Cell Line Cloning, Molecular DNA Restriction Enzymes/metabolism DNA, Viral/analysis Gene Expression Regulation Hepatitis B Surface Antigens/genetics Hepatitis B virus/genetics Humans Liver Neoplasms Microscopy, Electron
Chemicals
DNA, Viral Hepatitis B Surface Antigens DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dejean A
Brechot C
Tiollais P
Wain-Hobson S
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.9.2505
Published
1983-05-00
Pages
2505-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393854
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.9.2505 PMC Full Text View journal details
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