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PMID: 350830 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stable denaturation of chromosomal DNA from Saccharomyces cerevisiae during meiosis.

Journal of bacteriology ·Vol. 134 ·No. 2 ·1978-05-00 ·Pages 629-35

Klein HL, Byers B

Abstract

Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time course of their appearance indicated that the denatured regions were present during the periods of premeiotic DNA replication and recombination. A similar pattern of denaturation was also detected in the DNA from vegetatively grown cells of a conditional cdc8 mutant, which is defective in DNA replication.

MeSH Terms
Chromosomes/metabolism DNA/metabolism DNA Replication Meiosis Microscopy, Electron Mitosis Mutation Recombination, Genetic Saccharomyces cerevisiae/cytology,metabolism Temperature
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klein H L
Byers B
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30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.2.629-635.1978
Published
1978-05-00
Pages
629-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222296
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.2.629-635.1978 PMC Full Text View journal details
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